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Sarcomeres in cardiac myocytes (heart muscle cells) are mechanically coupled to focal adhesions through dorsal stress fiber-like structures. #cardiotwitter #CellBiology
1/13
A thread based on Figure 1
A mature adult cardiac myocyte is packed with sarcomeres, whose contractile forces are coupled to the extracellular environment. With sarcomeres so close to the plasma membrane, how can we study the nature of this coupling?
2/13
Short answer: find a model system where the sarcomeres are not so close to what the cardiac myocyte is attached to. Enter, iPS cell-derived cardiac myocytes. These are “immature” in culture as they resemble fetal or neonatal cardiac myocytes.
3/13
Our previous work on iPS cardiac myocytes reported that sarcomere containing myofibrils assembled on the top surface of the myocyte.
https://t.co/xIBCu3hG1W
4/13
The sarcomeres seemed to be connected to focal adhesions on the bottom of the cell by thin actin bundles that resembled the dorsal stress fibers (DSF) commonly found in non-muscle cells. This movie steps through a Z stack of a myocyte starting at the bottom of the cell.
5/13
1/13
A thread based on Figure 1
A mature adult cardiac myocyte is packed with sarcomeres, whose contractile forces are coupled to the extracellular environment. With sarcomeres so close to the plasma membrane, how can we study the nature of this coupling?
2/13

Short answer: find a model system where the sarcomeres are not so close to what the cardiac myocyte is attached to. Enter, iPS cell-derived cardiac myocytes. These are “immature” in culture as they resemble fetal or neonatal cardiac myocytes.
3/13

Our previous work on iPS cardiac myocytes reported that sarcomere containing myofibrils assembled on the top surface of the myocyte.
https://t.co/xIBCu3hG1W
4/13

The sarcomeres seemed to be connected to focal adhesions on the bottom of the cell by thin actin bundles that resembled the dorsal stress fibers (DSF) commonly found in non-muscle cells. This movie steps through a Z stack of a myocyte starting at the bottom of the cell.
5/13

1. -Teeth Symbolism comms.
If something "has teeth" it's effective
If it was "like pulling a tooth" it required effort to do.
Thus in comms it's used for plants working together and removal of dug-in plants.
e.g. S. Rich died July 2016
https://t.co/l9eUti2Wq9 symbolism signal
If something "has teeth" it's effective
If it was "like pulling a tooth" it required effort to do.
Thus in comms it's used for plants working together and removal of dug-in plants.
e.g. S. Rich died July 2016
https://t.co/l9eUti2Wq9 symbolism signal
1. - Tooth Extracted Celebration Comms.
— CodesUcq (@CodesUcq) September 18, 2020
09/16/2020 Dentist Seth Lookhart extracted tooth July 2016 on hoverboard sentenced to 12 years
Note cheer after extraction in pic 3.
Does my expanding the article title give a clue as to the meaning? Yes it does.https://t.co/nOtO1DhnGy