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NotTheMacAnon
@NotTheMacAnon1 5 years, 8 months ago 1122 views

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This nonspecific cation channel opens when illuminated with blue light, depolarizing the cell [4, 6] to trigger action potentials cells with high temporal precision [5]. Since the initial discovery of ChR2, many modifications have been made to refine the kinetic properties.

For example, ChETA, engineered to address limitations of spike fidelity with ChR2, has faster temporal kinetics and can maintain reliable spike precision up to 200 Hz [7].

Halorhodopsins (NpHR), light-gated chloride pumps activated by yellow light, allow for temporally specific hyperpolarization with single spike precision [8–10].

Archaerhodopsin-3 (Arch), a yellow light-activated outward proton pump, is an increasingly popular tool for light-induced hyperpolarization to inhibit cellular activity. Arch recovers spontaneously from inactivation with a much shorter recovery time than NpHRs [11].

Arch currents continue to increase with increasing light intensity, while NpHRs saturate [11]. The increased light sensitivity of the ArchT variant, along with better membrane targeting, allows for improved neural silencing both at cell bodies and terminals [12].

Many more tools have been engineered with unique optical properties and kinetics. Step-function opsins (SFOs) are bistable ChR2s that can maintain a stable open confirmation to induce a step in membrane potential [13].

Opsins with shifted excitation wavelengths allow for differential cellular activation. Chrimson, a red-shifted opsin, can be used in combination with Chronos, a blue-green activated channel with increased light sensitivity and faster kinetics,

to achieve two-color activation of distinct neuronal populations [14]. Since proton pumps are relatively inefficient compared to channels, moving only one ion across the membrane per photon rather than a steady current of ions, to achieve improved neural silencing,

ChR2 was genetically engineered to conduct chloride ions (Cl−) to create a depolarizing channel [15]. Further modifications generated step-function control and improved light sensitivity and kinetics, making these useful tools for controlling behavior in freely moving animals

I will hold my tongue.

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NotTheMacAnon
@NotTheMacAnon1

Transduction is the process by which foreign DNA is introduced into a cell by a virus or viral vector.

Viral vectors are tools commonly used by molecular biologists to deliver genetic material into cells.

Viruses have evolved?

specialized molecular mechanisms

to efficiently

transport their genomes

inside the cells they infect.

Delivery of genes, or other genetic material, by a vector is termed transduction and the infected cells are described as transduced.

A transducer is a device that converts energy from one form to another. Usually a transducer converts a signal in one form of energy to a signal in another.

NotTheMacAnon
@NotTheMacAnon1

So where are we at with this virus people?

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NotTheMacAnon
@NotTheMacAnon1

In continuum mechanics, stress is a physical quantity that expresses the internal forces that neighbouring particles of a continuous material exert on each other, while strain is the measure of the deformation of the material.

For example, when a solid vertical bar is supporting an overhead weight, each particle in the bar pushes on the particles immediately below it. When a liquid is in a closed container under pressure, each particle gets pushed against by all the surrounding particles.

The container walls and the pressure-inducing surface (such as a piston) push against them in (Newtonian) reaction.

These macroscopic forces are actually the net result of a very large number of intermolecular forces and collisions between the particles in those molecules. Stress is frequently represented by a lowercase Greek letter sigma (σ).

Residual stresses inside a plastic protractor are revealed by the polarized light.

NotTheMacAnon
@NotTheMacAnon1

Localized Surface Plasmon Resonance - an overview | ScienceDirect Topics

https://t.co/mzS7vVSREJ

https://t.co/353PdAX2fa

https://t.co/3yBImjOdd4

In some cases, almost 100% of the light energy can be converted to the second harmonic frequency. These cases typically involve intense pulsed laser beams passing through large crystals, and careful alignment to obtain phase matching.

NotTheMacAnon
@NotTheMacAnon1

2020 marks the start of the quantum dot revolution.

Completely ignoring the fact that "photocatalytic generation of reactive oxygen species (ROS) from quantum dots (QDs) has been widely reported."

Results: It was found that whereas singlet oxygen (1O2) was not produced by photoexcited QDs, superoxide anion(O2− )and hydroxyl radicals(OH) were generated by QDs, especially in the presence of a physiological concentration of electron donating agent including NADH.

Using cell metabolic activity assays and various probes of ROS generation, the formation of ROS in cellular environments was demonstrated.

Conclusion: Illumination of QD-treated cells and bacteria with light did affect viability. The above results, together with those of assays in the presence of various scavengers of specific ROS, indicate that the formation of ROS by QDs mainly proceeded via electron transfer.

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Simon Wilson
@simonwilson2304

I'm married to an Australian. I returned to Australia from the UK in October.

This is my experience of what happened to me when I arrived in the country during COVID.

I think it will help people understand how criminally bad our government's response has been. (a thread).

On arrival, passengers were escorted from the plane by armed police.

We were allocated a government-mandated hotel, and told to wait in a holding area.

Once processed we were led to a coach. Members of the Australian army lined the route to make sure no one tried to escape.

Once at the hotel we were taken off the coach one by one

An Australian police officer escorted me to my room, telling me in no uncertain terms that if I left it in any unauthorised circumstances for the next two weeks, I would be spending up to two years in an Australian prison.

During the next two weeks I was fed three times a day in polystyrene and with plastic cutlery. Below are some examples of the food. It was fine, but there was no control or choice.

After about eight days I was tested for COVID.

This was mandatory, and the first time I’d seen another human since the quarantine started.

Even though I was negative, I was told I could leave only once my 14 days of isolation were over.

$\uc2e0\ub150\uc758\uc0ac\ub098\uc774$

신념의사나이...
@Belposto_korea

The Transition from the old to the new.

The QFS is alive and doing its snoopy thing, but it is not yet active with accounts. How is the old going to transition to the new? This is a good question./1

The QFS must be active when the RV/Redemption takes place because all RV and Redemption funds will be placed in the QFS as gold-back currencies in the currency of the nation where the RV or the Redemption takes place./2

This is the Global Currency Reset that all countries will go through when the funds are released. The RV funds are released and deposited into QFS accounts as gold-backed currencies; thus the Global Currency Reset./3

Keep in mind that this Global Currency Reset means the world has reset their currencies to the whole new system of finance for the world.
There will be no fanfare or public announcement when the QFS is activated./4

There are those in the financial industry who will know or suspect that the QFS has been turned on but there will be very few “tells” that it has been activated and fully functioning. The alliance wants this to be seamless./5

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Kab
@Kabamur_Taygeta

#NEIOH

1. Friends Of The Great Planet Earth!

Everything You Seek Is Already Within You! Each Waking Dream Has False Teachings That Say There Is A Special Place 'Out There' In The Skies Where People Go That Follow A Certain Doctrine.

2. And Those Same Teachers Explain There Is A Place Of Torment For Others That Arrived On A Different Part Of The Planet And Were Taught A Different Doctrine.

There Is ONE Creator That Has Been Named By Many. It Would Be Loving To Honor And Respect Another's Beliefs,

3. As In Truth, Many Have Lived In Multiple Cultures With Many Beliefs That Now Are Judged! Incarnating Over And Over Is The Joy Of The Soul Experiencing Life! The Soul Fully Understands This Is A Human Experience.

4. Wouldn't It Be Much More Relaxing And Fun To Allow Others Their Freedom, Just As You Want Yours? Yes Indeed! Relax And Flow!

Since The Very Beginning Of Your Self-Awareness As An Incarnate Being, You Have Probed Your World For Clues To The Meaning Of Your Existence.

5. You Have Looked In Every Conceivable Crevice And Pondered The Vastness Of The Infinite Cosmos. You Have Scrutinized The Spiritual Wisdom That Was Forthcoming From Those Who Believed Themselves To Be Knowledgeable About Such Matters.

Catherine Griwkowsky
@CGriwkowsky

Welp, I have no sound on the phone line for this news conference, so I shall tune in via video. Kenney is speaking and there is a photo of the boreal region.

Lost video too! Before that cut out, Kenney said they are expanding the Kitaskino Nuwenëné Wildland Provincial Park.

Back on the phone line. Environment and Parks Minister Jason Nixon. Athabasca Oil surrendered 23,500 acres in that region. Cenovus surrendered more than 60,000 acres.

Chief Peter Powder, Mikisew Cree First Nation, says we need to protect the watershed, the caribou habitat. Expansion of the Kitaskino Nuwenëné Wildland Provincial Park in the Athabasca Delta will protect most vulnerable species, he says

Powder thanks the federal government for financial support for the parks and thanks the province for supporting the vision.

silkcoeur
@silkcoeur

[🔞 hopekook, rimming] happy vday if you celebrate it, last night i fell asleep thinking about a horny hopekook date where they set aside time to cuddle and watch something soothing and mindless. jk chooses a nature documentary, more for background ambiance than anything else.

they start out with hobi laying crosswise on the couch, and jk crawls between his legs, rests his head on hobi's sternum. he likes to cuddle like that because he can hear hobi's heartbeat. also hobi will usually skritch his scalp when they're in this position.

he's doing that now, running his fingers from jk's forehead to the nape of his neck, but then he scoops along jk's ear instead. fingertips smoothing along the shell curve and then gently tugging on his earlobe. he keeps doing it, repetitive. the apartment is warm.

and quiet, too, except for the doc in the bg and the soft sounds of their breathing. hobi's fingertips keep tugging easily, gently on his ear lobe and all of a sudden jk realizes he's getting hard. he doesn't pay it much mind, one or both of them often do when they cuddle, even

if it's just a cuddle session & not anything further. but then hobi moves his leg, feels it, laughs softly, & moves his leg again - this time, deliberately. jk smiles, rubbing his mouth against hobi's sweatshirt. he rolls his hips, real slow like, and feels hobi's dick, too.

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Latest update from @TheBernician

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2/19

The four defendants will be Matt Hancock, Chris Whitty, Patrick Vallance and Neil Ferguson, each of whom has inextricable and ultimately incriminating connections with Bill Gates and Big Pharma.

3/19

As well as three counts of fraud by false representation and seven counts of fraud by non-disclosure, under sections 2 and 3 of the Fraud Act 2006, we can now also prove that the entire worldwide scamdemic originated on these shores.

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In fact, we can show that, without the dishonest statements and non-disclosures of the four defendants we are initially proceeding against, COVID-1984 would not have transpired.

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Marvels of Medical Science in Ancient India

ACHARYA SUSHRUTA and SUSHRUTA SAMHITA

Ancient Indians were pioneers in diverse fields of study and their genius was centuries ahead of their times. Feel the glory, basking in pride of their unparalleled achievements.

(Thread)

Sushruta (circa 7th or 6th century BC) was a surgeon who in present times is known as the “father of Indian medicine” and “father of plastic surgery” for inventing and developing surgical procedures. He lived in Kashi on the banks of river Ganga.(1)

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Mitesh Patel
@Mitesh_Engr

How to sell option?
Here let me explain through simple math.
Say BN at 30000
30000 straddle trading at combine premium 800 ( based on current volatility)
Put at 400
Call at 400
You don’t know direction. Sold both.

If move will not come than both will decrease gradually.

At day end it will come down to 720-740 if move will not come. Difference is your profit.

Now case 2:
Move started.
At one points combine premium will start increasing.
Say 810.
Exit immediately. No need to do adjustment.
Let market settle.

Sell again straddle once market settle.
Now repeat same thing.

Straddle selling webinar over.
Good luck.

One can keep SL 550 for both strike to escape from sudden movement.

Anastasis
@OrdersofM

https://t.co/6cRR2B3jBE
Viruses and other pathogens are often studied as stand-alone entities, despite that, in nature, they mostly live in multispecies associations called biofilms—both externally and within the host.

https://t.co/FBfXhUrH5d

Microorganisms in biofilms are enclosed by an extracellular matrix that confers protection and improves survival. Previous studies have shown that viruses can secondarily colonize preexisting biofilms, and viral biofilms have also been described.

...we raise the perspective that CoVs can persistently infect bats due to their association with biofilm structures. This phenomenon potentially provides an optimal environment for nonpathogenic & well-adapted viruses to interact with the host, as well as for viral recombination.

Biofilms can also enhance virion viability in extracellular environments, such as on fomites and in aquatic sediments, allowing viral persistence and dissemination.