BC SCIENCE
Saved by @CodyyyGardner

Let Uncle Ralph educate you
Synthetic Viral Genomics:
Risks and Benefits for Science and Society

 "Alternatively, “No See’m” sites can be used to insert foreign genes into viral, eukaryotic, or microbial genome or vector, simultaneously removing all evidence of the restriction sites that were used in the recombinant DNA manipulation"
By orientating the restriction sites as “No See’m”, the sites are removed during reassembly, leaving only the desired mutation in the final DNA product.
The dual properties of strand specificity & a variable end overhang that can be tailored to match any sequence allow for Esp3I sites to be engineered as “universal connectors” that can be joined with any other four nucleotide restriction site overhangs (e.g. EcoRI, PstX1, BamH1)
Seamless assembly (also called No See’m Sites (85)) cascades have been used to assemble full length cDNAs of the coronaviruses mouse hepatitis virus, transmissible gastroenteritis virus, infectious bronchitis virus and SARS-CoV (Refs: 85,86,87)
Type IIS restriction endonucleases recognize asymmetric binding sites & leave asymmetric ends
These enzymes can be used to create unique interconnecting junctions, which can be subsequently removed from final assembly product allowing seamless reconstruction of an exact sequence
With enzymes like Esp3I, interconnecting restriction site junctions can be located at ends of each cDNA & systematically removed during assembly of complete full-length cDNA product
Consequently, knowledgeable experts can theoretically reconstruct full length synthetic genomes for any of the high priority virus pathogens, although technical concerns may limit the robustness of these approaches.
Another approach might be to “humanize” zoonotic viruses by inserting mutations into virus attachment proteins or constructing chimeric proteins that regulate virus species specificity (viral attachment proteins bind receptors, mediating virus docking and entry into cells).
The pathogenicity of these chimeric coronaviruses is unknown
The pathogenicity of these chimeric coronaviruses is unknown
The pathogenicity of these chimeric coronaviruses is unknown
The pathogenicity of these chimeric coronaviruses is unknown
"Scapegoat option"
This powerful technique provides bioterrorists with a “scapegoat” option; leaving a sequence signature that misdirects efforts at tracking the true originators of the crime. Even better, the approach could be used to build mistrust &/or precipitate open warfare
Dual Use
Uncle Ralph summarises his findings (1)
Uncle Ralph Concludes (2)
Hence Ecohealth DARPA/DTRA spooks & virus thieves collaboration with Baric (UNC) Lipkin (Mailman) NIchols (Atlanta CDC) and USAMRIID (Bavari, Totura et al) & Jonathan Epstein's palpable concern about dual use references in the @USRightToKnow FOIA emails
A scary read for an experimental monkey!
Delving into the cold and calculating mind of a twisted genius?
https://t.co/MrwxYK93cx
Bring Uncle Ralph and his transgenic mice in for questioning!
unroll @threadreaderapp
You just read the Baric Paper, one of 6 in a series, the rest are available here in a 191 page document
https://t.co/rjNZ8aDNDl
Related Papers
1. Synthetic Genomics: Options for Governance (2008) https://t.co/A32jEfL1TQ
2. Sequence Screening - Robert Jones (2005)
https://t.co/zOqMroYbVU
3. Synthetic Biology as a Field of Dual-Use Bioethical Concern - Alexander Kelle
https://t.co/Va1movPqvs
Related papers (2)
4. Sanghvi Y. A Roadmap to the Assembly of Synthetic DNA from Raw Materials.
https://t.co/4kWu4vzcXC
5. Collett MS. Impact of Synthetic Genomics on the Threat of Bioterrorism with Viral Agents.
https://t.co/OcaTmuCUBy
Related Papers (3)
6. Fleming DO. Risk Assessment of Synthetic Genomics: A Biosafety & Biosecurity Perspective.
https://t.co/JBcLVLr005
7. Risk Governance of Synthetic Biology
https://t.co/9BE9X5s0Fb
8. US Competitiveness in Synthetic Biology
https://t.co/OCZJdsXSjl
Related papers (4)
9. Ensuring security of synthetic biology
https://t.co/7kPE1OpbrS
10. Synthetic biology: emerging research field in China
https://t.co/T3BcaDvUsa
11.
What rough beast? Synthetic biology, uncertainty,& the future of biosecurity (2016)
https://t.co/XTlBXILEWh
Back to Baric et Al
Reverse genetics with a full-length infectious cDNA of severe acute respiratory syndrome coronavirus (2003)
https://t.co/k8PI1rbcm8
Systematic Assembly of a Full-Length Infectious Clone of Human Coronavirus NL63 (2008)
https://t.co/bWhAv1wsC0
For the Record (WIV)
In 2016, Shi and her team at the WIV, in conjunction with the New York-based EcoHealth Alliance, constructed a full-length clone of a bat coronavirus called SL-CoV WIV1. They assembled it in discrete segments.
They genetically engineered the virus using the pGEM®-T Easy Vector Systems to join the segments. This system, also available on the internet, gives researchers several options for how to remove GM inserts that can be seen as signatures of a lab-made virus.18
pGEM®-T Easy Vector Systems:
“Thus, several options exist to remove the desired insert DNA with a single restriction digestion.”
This shows that researchers at the WIV have the ability to genetically engineer viruses and remove the signatures of the genetic engineering.
WIV & EcoHealth Alliance published a paper in 2017 on how they genetically modified spike proteins of 8 bat coronaviruses, by cutting & pasting genetic material from other coronaviruses, so that the viruses infected the human ACE2 receptor
They used pGEM®-T Easy Vector Systems to join the segments to genetically engineer these viruses.
They showed how they can insert new spikes into viruses. The researchers state:
“Then any spike could be substituted into the genome of SARSr-CoV WIV1 through this strategy.”
This shows that researchers at WIV have the ability to genetically modify multiple coronaviruses to insert new spikes, and these new viruses cannot be detected as genetically engineered.
The research clearly shows that GOF researchers at WIV could assemble SARS-CoV-2 from bat coronaviruses, such as RaTG13 or similar & spike protein from Malayan Pangolins & insert multi-basic cleavage sites into precise regions of spike & leave no evidence of genetic engineering.
All the above about WIV research is taken verbatim from an excellent article by Andre Leu on the website of @OrganicConsumer
COVID 19: The Spike and the Furin Cleavage
https://t.co/bDOMmhpJ1t

More from Billy Bostickson 🏴👁&👁 🆓

Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193
Billy Bostickson 🏴👁&👁 ...
@BillyBostickson
1/X Bellagio Rockefeller Initiative (Global Virome Project)
Another look at some key people in the GVP & their media statements since January 2020 about "natural origin" and impossibility of a "lab leak"
Full List of 18 scientists in photograph is here:
https://t.co/yHoAjwQjI8


2/X
We have already looked closely at Dennis Carroll, Peter Daszak, George Gao, W. Ian Lipkin & seen their public statements supporting "natural origin" & hysterical attacks on "lab leak" theories.
This thread focuses on:
Michael Kurilla
Jonna Mazet
Edward Rubin (Metabiota)

3/X
If we look at the leadership team of GVP, you will see some familiar faces:
https://t.co/WdT8sNnfmy
Most familiar is probably Peter Daszak, the wealthy Treasurer, also with the key role of Secretary.
Note: USAID PREDICT Project - EcoHealthAlliance


4/X
The "Chair" is Dr. Dennis Carroll
We will have a look at his media statements on the coronavirus pandemic by inputting his name in Twitter search box (very easy!)
Note: Previously was Director of USAID Emerging Pandemic Threats Program


5/X Dennis Carroll's video media statements (1):
CNN
(note Qinghai Thangka positioned in background which Daszak also shows off in his videos obtained during EcoHealth virus hunt with
ALL
Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193
Billy Bostickson 🏴👁&👁 ...
@BillyBostickson
An "Aquarium" needs cleaning in my opinion, but who am I, a mere monkey, to give advice?
Astonished at what @bellingcat discovered about GRU & FSB operations
Skripal https://t.co/PzH3a7JZvy
Navalny https://t.co/eZi63UhWw1
FSB https://t.co/sViYk9RwmB
GRU https://t.co/MY5d5gw2OG


A great post by Ilya Yahin on

Every time Putin talks about “our special services,” his signature smirk appears on his face, and in his words there is poorly concealed bragging
"Of course, we knew everything in advance"
"Yes, we are looking"
"Mercader has already been sent"
“World-class professionals”

Putin clearly loves all these spy games. He is very proud of the special services and therefore endlessly increases budget funding for them.
This is his top priority.
The elderly dictator cannot understand one thing:

there are no effective special services in a state that is completely corroded by corruption. You won't have honest professionals at your fingertips if your son-in-law buys shares of a state company for $ 100, the real value of which exceeds $ 380 million.
ALL , BUSINESS
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Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193
Billy Bostickson 🏴👁&👁 ...
@BillyBostickson
1. WIV Research groups (archived pages) for Reference
dsRNA viruses molecular biology

https://t.co/lwrQoo6ygG


2. Emerging Viruses Group

https://t.co/ND56gVGOAn


3. Structural Biology of Viral Genome Replication
https://t.co/wUqc0YIP3X


4. Molecular Biology of hepatitis Viruses & Gene Therapy
https://t.co/snBbxkhNsC


5. Insect Virus Genetic Engineering Lab
https://t.co/S68mVOA2Ob
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Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193
Billy Bostickson 🏴👁&👁 ...
@BillyBostickson
1/ 10
My Tedious Research Thread on Wuhan Seafood Market Positive Environmental Samples and possible links to Animals
Search query: "market" "animal" on cloned github database (deleted on github due to CCP) here:

29/30
Hubei Daily (2015) reported that Zhou Zhanhong, former party group member & chief economist of Wuhan Municipal Administration of Industry & Commerce was arrested on suspicion of abuse of power & bribery, but no trial information has been seen
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Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193
Billy Bostickson 🏴👁&👁 ...
@BillyBostickson
@KeithEv84928885 @threadreaderapp @buzz_chronicles @UnrollThread you should carefully prune it and create a report and publish it on researchgate!
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Corey Lynn \U0001f64f\U0001f1fa\U0001f1f8
Corey Lynn 🙏🇺🇸...
@CoreysDigs
1) OBSERVATION: The frequencies are changing. I would have to assess stats on the electromagnetic field to see its fluctuations. All I know is, I am highly sensitive to energy/frequencies, and they are changing, because…

2) 2 Weeks ago, my dog was effected by it as well. He is hearing things that aren’t technically audible. I can tell by his body language and sounds. One week ago I began feeling a pressure in my head and ears. It wasn’t painful at all, but it was intense for a bit.

3) It reminded me of taking off in an airplane a bit. I felt like I was in a vacuum. It went on and off for a couple of days. I’ve picked up on frequencies and high pitched sounds (not audible to most) for 30 years, and today my right ear began to ring for longer than usual.

4) This is nothing unusual for me, with exception of the duration. This happens often with me. No, it’s not tinnitus. Only this time, when I spoke, there was reverb creating a high pitch in my ear from my voice. This lasted for about 30 minutes. It did not hurt, just annoying.

5) So yes, the frequencies are changing. What this means exactly, I am unsure of at the moment. Some will say electromagnetic fields, others will say ascension, and some will say pole shift. I honestly can’t say at this very moment.
SCIENCE
10-K Diver
10-K Diver
@10kdiver
1/

Get a cup of coffee.

In this thread, we'll cover *State Quantities* and *Flow Quantities*.

This mental model can help us analyze many different entities -- from complex engineering systems to publicly traded companies.


2/

A "State Quantity" is something that's associated with a *specific point in time*.

For example, the number of followers a Twitter account has is a state quantity. This number can go up and down over time. But at any one *specific* time point, it's fixed.

3/

Similarly, the amount of cash a company has is a state quantity.

This cash balance can also rise and fall over time.

But pick any one *specific* time point -- and the company can only have one *specific* cash balance at that time point.

4/

By contrast, a "Flow Quantity" is something that's associated with a time *interval* rather than a time *point*.

For example, the number of followers gained by a Twitter account is a flow quantity. It's meaningful only when we specify a time interval (eg, the last 30 days).

5/

A simple analogy is that a State Quantity is like a photo -- a snapshot at a particular *point* in time.

But a Flow Quantity is like a video -- a record of events that take place during a particular time *interval*.
SCIENCE , FINANCE
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agamshastra
agamshastra
@truejainology
Some 1300 years ago in Karnataka there lived a genius who wrote "Gaṇitasārasan̄graha" (which is earliest text devoted entirely to mathematics)

The man who's work later borrowed by Arabs & Europeans

Thread on "Mahāvīracharya" astounding but forgotten legacy


Mahāvirachārya was patronised by rashtrakuta king amoghavarasha

His book"Gaṇita-sāra-saṅgraha"is earliest text devoted entirely to Mathematics

He has described the currently used method of calculating(LCM)of given numbers.The same method was used in Europe later in1500CE

Mahavira wrote "Gaṇita-sāra-saṅgraha" (GSS) in 8th century CE which contains some 1100 slokās & contains elementary topics in arithmetic , algebra , geometry , measurements , logic , number theory , dynamical systems etc

A sheer mathematical genius


He was devout jain
His magnum opus "Ganit sara sangraha " start with a sloka bowing to the glory of the jinas

"संख्याज्ञानप्रदिपेन जैनेंद्रन महत्वेशा,
प्रकाशितम जगतसर्व येन तम प्रणमाम्यहम"

He also praised his predecessor aryabhata , brahmagupta & other greats in his works


The importance & popularity of "Gaṇita-sāra-saṅgraha"was so huge that it enjoyed statues of most important textbook of mathematics in South Indian for over 3 centuries

A large no of manuscript copies of
"Gaṇita-sāra-saṅgraha"whr discovered in Kerala shows it's popularity
SCIENCE
Lior Pachter
Lior Pachter
@lpachter
"The new answer to a 77-year-old problem"

😭


https://t.co/hm9NoaU4nr


https://t.co/8fKDiKjSWc


https://t.co/jkaicC1F2x


https://t.co/PpxWT4Jef4
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Thomas A. Fine \U0001f1fa\U0001f1f8
Thomas A. Fine 🇺🇸...
@thomasafine
Let's talk about Avi Loeb and his theory about alien

First, I should disclose that I work at the fame research facility as Dr. Loeb, though I work in a different department and to my recollection we've never met.

He's a scientist; I'm in computer support.

With that out of the way...
The interview above is worth a read, and a lot of serious thought, because there's an idea there that's really critical to science, and it isn't whether or not aliens have visited (exactly).

It's about how fashion and culture and taboo have an unfortunately strong effect on science.

In particular he compares his theory on alien visitation with the multiverse theory.

Which of these is more deserving of ridicule?

Science is fundamentally a structure for forming hypotheses and then (critically) testing these hypotheses to see if they should be kept, rejected, or modified.
SCIENCE

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Unfortunately the "This work includes the identification of viral sequences in bat samples, and has resulted in the isolation of three bat SARS-related coronaviruses that are now used as reagents to test therapeutics and vaccines." were BEFORE the


chimeric infectious clone grants were there.https://t.co/DAArwFkz6v is in 2017, Rs4231.
https://t.co/UgXygDjYbW is in 2016, RsSHC014 and RsWIV16.
https://t.co/krO69CsJ94 is in 2013, RsWIV1. notice that this is before the beginning of the project

starting in 2016. Also remember that they told about only 3 isolates/live viruses. RsSHC014 is a live infectious clone that is just as alive as those other "Isolates".

P.D. somehow is able to use funds that he have yet recieved yet, and send results and sequences from late 2019 back in time into 2015,2013 and 2016!

https://t.co/4wC7k1Lh54 Ref 3: Why ALL your pangolin samples were PCR negative? to avoid deep sequencing and accidentally reveal Paguma Larvata and Oryctolagus Cuniculus?
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Here I will share what I believe are essentials for anybody who is interested in stock markets and the resources to learn them, its from my experience and by no means exhaustive..

First the very basic : The Dow theory, Everybody must have basic understanding of it and must learn to observe High Highs, Higher Lows, Lower Highs and Lowers lows on charts and their

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THE EVOLUTION OF OUR NATIONAL FLAG (1857-1947)

The INDIAN NATIONAL FLAG is not just a flag! It's a testimony of patriotism and seamless sacrifice. A symbol representing one of the oldest civilizations on this planet.

Let's hope what happened yesterday never repeats!

(Thread)


After the revolt of 1857, the first Indian flag was designed. The design was based on western heraldic standards with "Union Jack" in the upper-left quadrant and a "Star of India" enclosed in the royal crown in the middle of the right half.(1)


The flag was a symbol of the direct imperial rule in India.(2)

In 1904 the first Indian flag which stood for India's independence from the British was devised. The design was provided by sister Nivedita, an Irish disciple of Swami Vivekananda.(3)


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