https://t.co/LjpdlHa0C7 is getting a good face-lift.
Samples now have variant tables with annotation on their impact to the gene.
There is also an IGV integration. You need IGV installed locally but the Start|Jump links will open up you BAM file in IGV.

You must have IGV open and then click the start link.
The sample's BAM will show up for viewing in IGV.
Once open, you can use the jump link to move swiftly around to each variant. This is a variant in THCAS.
You can jump to other variants in the same gene. In this case we have one non-synonymous variant in phase with a synonymous variant in THCAS.
Cannabinoid synthase genes are cute but some folks can answer those questions with HPLC.
How about flowering genes?

This sample has a disruptive in frame deletion on a late flowering gene. And it's very rare in the population.
Looks to be heterozygous....
But its never this simple in cannabis.

Let's look at what other damaging variations it has.
7 Damaging inframe deletions or insertions (indels) in Early and Late flowering genes.
When it comes to Indels, IGV can be very helpful to inspect the read frequency and if there appears to be any read mismapping with short reads. They all look real and diploid.
Here is another one in AAE1-2. This gene is involved in making the pre-cursors to cannabinoid synthesis.

It is not just a simple presence or absence of the cannabinoid synthase genes. One has to pay close attention to variants up stream in the pathway as well.
@threadreaderapp unroll

More from Tech

1. One of the best changes in recent years is the GOP abandoning libertarianism. Here's GOP Rep. Greg Steube: “I do think there is an appetite amongst Republicans, if the Dems wanted to try to break up Big Tech, I think there is support for that."

2. And @RepKenBuck, who offered a thoughtful Third Way report on antitrust law in 2020, weighed in quite reasonably on Biden antitrust frameworks.

3. I believe this change is sincere because it's so pervasive and beginning to result in real policy changes. Example: The North Dakota GOP is taking on Apple's app store.


4. And yet there's a problem. The GOP establishment is still pro-big tech. Trump, despite some of his instincts, appointed pro-monopoly antitrust enforcers. Antitrust chief Makan Delrahim helped big tech, and the antitrust case happened bc he was recused.

5. At the other sleepy antitrust agency, the Federal Trade Commission, Trump appointed commissioners
@FTCPhillips and @CSWilsonFTC are both pro-monopoly. Both voted *against* the antitrust case on FB. That case was 3-2, with a GOP Chair and 2 Dems teaming up against 2 Rs.

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@franciscodeasis https://t.co/OuQaBRFPu7
Unfortunately the "This work includes the identification of viral sequences in bat samples, and has resulted in the isolation of three bat SARS-related coronaviruses that are now used as reagents to test therapeutics and vaccines." were BEFORE the


chimeric infectious clone grants were there.https://t.co/DAArwFkz6v is in 2017, Rs4231.
https://t.co/UgXygDjYbW is in 2016, RsSHC014 and RsWIV16.
https://t.co/krO69CsJ94 is in 2013, RsWIV1. notice that this is before the beginning of the project

starting in 2016. Also remember that they told about only 3 isolates/live viruses. RsSHC014 is a live infectious clone that is just as alive as those other "Isolates".

P.D. somehow is able to use funds that he have yet recieved yet, and send results and sequences from late 2019 back in time into 2015,2013 and 2016!

https://t.co/4wC7k1Lh54 Ref 3: Why ALL your pangolin samples were PCR negative? to avoid deep sequencing and accidentally reveal Paguma Larvata and Oryctolagus Cuniculus?
One of the most successful stock trader with special focus on cash stocks and who has a very creative mind to look out for opportunities in dark times

Covering one of the most unique set ups: Extended moves & Reversal plays

Time for a 🧵 to learn the above from @iManasArora

What qualifies for an extended move?

30-40% move in just 5-6 days is one example of extended move

How Manas used this info to book


Post that the plight of the


Example 2: Booking profits when the stock is extended from 10WMA

10WMA =


Another hack to identify extended move in a stock:

Too many green days!

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