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Saved by @BillyBostickson

https://t.co/OuQaBRFPu7
Unfortunately the "This work includes the identification of viral sequences in bat samples, and has resulted in the isolation of three bat SARS-related coronaviruses that are now used as reagents to test therapeutics and vaccines." were BEFORE the

 @franciscodeasis chimeric infectious clone grants were there.https://t.co/DAArwFkz6v is in 2017, Rs4231.
https://t.co/UgXygDjYbW is in 2016, RsSHC014 and RsWIV16.
https://t.co/krO69CsJ94 is in 2013, RsWIV1. notice that this is before the beginning of the project https://t.co/HPJhwq7NgH
@franciscodeasis starting in 2016. Also remember that they told about only 3 isolates/live viruses. RsSHC014 is a live infectious clone that is just as alive as those other "Isolates".
@franciscodeasis P.D. somehow is able to use funds that he have yet recieved yet, and send results and sequences from late 2019 back in time into 2015,2013 and 2016!
@franciscodeasis https://t.co/4wC7k1Lh54 Ref 3: Why ALL your pangolin samples were PCR negative? to avoid deep sequencing and accidentally reveal Paguma Larvata and Oryctolagus Cuniculus?
@franciscodeasis Ref.5: "However, inspection of Figure 4 shows that clade B is connected to viruses lacking T8782C and C28144T by single mutational steps via other human isolates, so this explanation requires not only positing two markets with two progenitors differing by just two mutations,
@franciscodeasis but also the exceedingly improbable evolution of one of these progenitors towards the other after it had jumped to humans."
@franciscodeasis in fact, both T8782C without C28144T and C28111T without T8782C have been found in humans. clearly these are impossible to be "two separate markets" as A and B have been found to be connected by single mutations in humans--requiring the exceedingly improbable event of one
@franciscodeasis progenitor evolving toward the other in humans.
@franciscodeasis https://t.co/4gQ4cwKyGk
@franciscodeasis As for ref 4? Why ALL the closest bat-borne backbones for SARS-CoV-2 LACKED the Spike that is necessary for infection of anything that isn't it's own species? RpYN06 had ZC45 S, which infect only R. Pusillus and R. Blythi. RpACE2 have been experimentally confirmed to be not
@franciscodeasis Usable by any RBD/RBM from the clade SARS-CoV or SARS-CoV-2, and could not have been able to recombine with any of these because of physical isolation between R. Pusillus and SARS-CoV-2-like Spike and RBD.
@franciscodeasis https://t.co/3vdIBEBA1k
@franciscodeasis Ref 6? Why the “full-length genomes” claimed by the RaTG15 paper were DISCONTIGUOUS with the RdRp sequences deposited under the title “Origin and cross-species transmission of bat coronaviruses in China” in 13-Aug-2019? https://t.co/gBfRmGcTf4 https://t.co/2E63NmRjTE
@franciscodeasis No other Betacoronaviruses have been isolated from R.blythi.
@franciscodeasis @garyruskin

More from Daoyu

Daoyu
Daoyu
@Daoyu15
@franciscodeasis https://t.co/Sd9IslUCH5 a FCS need a FCS in the inoculum to exist. It can not arise de-novo as it will be destroyed instantly by the immune system.

https://t.co/UgXygDjYbW a fourth Sars-like CoV is live at the WIV. This fourth virus is an infectious clone, where engineering of the S1-S2 is used regularly as mean to generate a culturable virus in HAE cells. No VERO E6 here, and HeLa-hACE2 is the new VERO of the WIV

https://t.co/DtjyycKy1v
https://t.co/PG7LVnHfsy
Even with VERO E6, only half the time does passage lead to the loss of the FCS—smaller plaques need to be explicitly picked for that to be a certainty.

Marburg virus is a novel virus that escaped from the lab. https://t.co/OGQM6qV27l the only reason why it did not become a pandemic is due to it being too lethal to sustain asymptomatic transmission in humans.

The highest reported number of cases were in WuChang right on top of the old WIV headquarters, In contrast to the population density data of Wuhan—note that the place near the market had the highest population density in all of Wuhan, which make it the most optimal location for
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Daoyu
Daoyu
@Daoyu15
@stevenemassey Only a sample is there. This cell line don’t have NGS. But it was consistent with a cell culture, and the RBD of the virus is not suitable for binding to the ACE2 found there. The ACE2 is same as Ra4331 and Ra9479.

And the RBD have a T403 that prevents infection of intestinal cells (hACE2 intestinal organoids, infection is restored upon T403R, but weaker than SARS-CoV-2) or lung cells (R.affinis primary, with supplemented hACE2. Infection is restored upon T403R).


Also that there exist no Sarbecovirus RBD with a D501.
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Daoyu
Daoyu
@Daoyu15
https://t.co/hXlo8qgkD0
Look like that they got a classical case of PCR Cross-Contamination.
They had 2 fabricated samples (SRX9714436 and SRX9714921) on the same PCR run. Alongside with Lung07. They did not perform metagenomic sequencing on the “feces” and they did not get


A positive oral or anal swab from anywhere in their sampling. Feces came from anus and if these were positive the anal swabs must also be positive. Clearly it got there after the NA have been extracted and were from the very low-level degraded RNA which were mutagenized from

The Taq. https://t.co/yKXCgiT29w to see SRX9714921 and SRX9714436.
Human+Mouse in the positive SRA, human in both of them. Seeing human+mouse in identical proportions across 3 different sequencers (PRJNA573298, A22, SEX9714436) are pretty straight indication that the originals

Were already contaminated with Human and mouse from the very beginning, and that this contamination is due to dishonesty in the sample handling process which prescribe a spiking of samples in ACE2-HEK293T/A549, VERO E6 and Human lung xenograft mouse.

The “lineages” they claimed to have found aren’t mutational lineages at all—all the mutations they see on these sequences were unique to that specific sequence, and are the result of RNA degradation and from the Taq polymerase errors accumulated from the nested PCR process
SCIENCE
Daoyu
Daoyu
@Daoyu15
https://t.co/TVXC4QOjt0
https://t.co/QnMiOrdNhx
https://t.co/ZK2vfwYEFj
in fact, adaptation in CaLu-3 actually reverses changes that happened in VERO E6. P681 and RRAR is fine-tuned to growth in CaLu-3 cell cultures. P681 guards the cardin-weintraub motif against cleavage in

cell lines.

https://t.co/vytn6YVRYQ
https://t.co/fjg6ZXc2KN
the FCS is perfectly stable in anything that isn't VERO E6 classic or 293T-ACE2. anything that had TMPRSS2 and grown in trypsin-free media stably maintains the FCS.

https://t.co/NUrJ8AndTx
in fact, the PRRARS, as opposed to other mutated cleavage sites--even the "perfect" H5CS--confers the greatest infectivity in CaLu-3 cells.

https://t.co/TVXC4QOjt0
in fact, even P681R or S686G changes were less fit in CaLu-3 compared to PRRA virus--the P681R virus show either no difference or is slightly less effective compared to the P681 virus, and the S686G virus
https://t.co/DNtIR17r4S
https://t.co/nDNNw9OaWd
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Daoyu
@Daoyu15
https://t.co/SvP0tfbDki


https://t.co/k9mmL9e5kn


https://t.co/7fR13yb3qJ


https://t.co/EXerlaiG9u


https://t.co/iNCdLY1Pzn
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Vibhu Vashisth
Vibhu Vashisth
@VIBHU_Tweet
RANI NAIKI DEVI AND THE BATTLE OF KASAHRADA

Mohammad Ghori, the young Ghurid Prince conquered Ghaznavids in Afghanistan in the year 1173 CE. He conducted some successful raids into the Indian territory, which even Mahmud Ghazni & the Persians couldn't do.


First&foremost,Ghori invaded states of Multan &Uch in India.After this he turned to southern states of Gujarat &Rajputana.Everybody knows this about Ghori,but what most pple r unaware of is the defeat he suffered at hands of Chalukyan Queen Naiki Devi in 1178 Battle of Kasahrada.


When he marched southwards, his first target was the prosperous fortified town of Anhilwara Patan.
It was the capital of Chalukyas(also known as Solankis of Gujarat) which was established by Vanraj of Chapotkata Dynasty in 8th century.


American historian Tertius Chandler states that it was the 10th largest city in the world at that time. The history of Chalukyas has been written by many historians, but the valour of the Great Queen Naiki Devi remains unsung.


Born to the Kadamba King Paramardin of Goa,Naiki Devi was well trained in sword fighting, horse riding, strategy & every other subject of Statesmanship. She was married to King Ajaypala of Gujarat who sat on throne in 1171CE. Unfortunately,the King died within 4 yrs of his rule.
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SK
SK
@sk_diary1
Kolaru Pathigam-Thevaram hymn composed by ThiruGnana Sambandar- #Thread

In Tamil Kol means planets,Aru means cut- set of songs in Kolaru Pathigam addresses all Navagrahas& last 2 lines of every song with end a plea to Bhagwan Shiva to reduce/erase the ill effects of Navagrahas


கோளறு பதிகம் - This pathigam was sung by Thirugnana Sambandar at Thirumaraikadu Kshetram -Vedaranyam,Tamilnadu

Thirugnana Sambandar & Appar(Thirunavukkarasar) travelled together to various Shiva Alayam to spread shivam & bhakti among people.They both reached Thirumaraikadu.


Thirumaraikadu(Vedaranyam) -
Appar & Sambandar were asked to enter the temple through side entrance.The main door was locked by Vedas many years ago.Appar&Sambandar sang devotional hymns praising Shiva, after which the gates opened & closed.After which main door opened for all.


After darshan of Bhagwan Shiva at Thirumaraikkadu,Sambandar received a message to travel to Madurai.
During that time, Samanam was on the rise in the Pandya Kingdom.The Pandya King converted to Samanam.The Queen Mangaiyarkkarasi was a staunch Shiva devotee and was upset.

Kularchirai Nayanar ,Chief Minister of Pandya kingdom was in agony by the turn of events.Queen requested Kulachirai Nayanar to escort Thiru gnana Sambandar to Madurai.Kularchirai Nayanar met Thiru gnana Sambandar at Thirumaraikkadu & requested him to visit Madurai.
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Ranvijay Singh\U0001f1ee\U0001f1f3
Ranvijay Singh🇮🇳...
@ranvijayT90
#Thread Two divine forms of Khatu Shyam ji...


On the day of Amavasya, devotees get the darshan of Shyam Baba in Shyam varan (black color). In ordinary days, the form of Baba is saffron. The reason for this is a custom. The real head of Shyam Baba which had appeared from Shyam Kund is of Shyam varan.


Every day Shyam Baba's grand Shringar is done by Pujaris. The main part of Shyam Baba's shringar is Tilak. This Shringar is done by the members of Nij Mandir sevaks. The sevaks of the Nij Mandir do sandalwood tilak to Baba everyday in the auspicious time.


This Divine Tilak is applied from the forehead to the nose. When Baba is given a bath by sevaks, this tilak comes off. Then in the auspicious time, the sevaks again apply tilak to Baba.


There is no auspicious time on the Amavasya. Because of this, tilak is not done on that day. That's why devotees have darshan of Shyam Baba on the Amavasya only in the actual real form (Shyam Swaroop). Baba's tilak is done on Amavasya only on the day of Deepawali.
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@buzz_chronicles @AchuthArora @OllieEmberson @rattibha No more missed tweet 😉!

I saved this tweet for you.

You must register on savetobookmarks .com in order to access and manage your bookmarks.
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Buzz Chronicles
Buzz Chronicles
@buzz_chronicles
@SaveToBookmarks Hello! There you go! Original tweet by @AchuthArora: "@Artofphysique_ @buzz_chronicles @rattibha @SaveToBookmarks save as Workouts". Cheers! 👌

Hi! Saving all night and all day :) Original tweet by @SaveToBookmarks: "@AchuthArora @Artofphysique_ @buzz_chronicles @rattibha Aye aye, captain👮‍♀️!

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Vibhu Vashisth
Vibhu Vashisth
@VIBHU_Tweet
दधीचि ऋषि को मनाही थी कि वह अश्विनी कुमारों को किसी भी अवस्था में ब्रह्मविद्या का उपदेश नहीं दें। ये आदेश देवराज इन्द्र का था।वह नहीं चाहते थे कि उनके सिंहासन को प्रत्यक्ष या परोक्ष रुप से कोई भी खतरा हो।मगर जब अश्विनी कुमारों ने सहृदय प्रार्थना की तो महर्षि सहर्ष मान गए।


और उन्होनें ब्रह्मविद्या का ज्ञान अश्विनि कुमारों को दे दिया। गुप्तचरों के माध्यम से जब खबर इन्द्रदेव तक पहुंची तो वे क्रोध में खड़ग ले कर गए और महर्षि दधीचि का सर धड़ से अलग कर दिया।मगर अश्विनी कुमार भी कहां चुप बैठने वाले थे।उन्होने तुरंत एक अश्व का सिर महर्षि के धड़ पे...


...प्रत्यारोपित कर उन्हें जीवित रख लिया।उस दिन के पश्चात महर्षि दधीचि अश्वशिरा भी कहलाए जाने लगे।अब आगे सुनिये की किस प्रकार महर्षि दधीचि का सर काटने वाले इन्द्र कैसे अपनी रक्षा हेतु उनके आगे गिड़गिड़ाए ।

एक बार देवराज इन्द्र अपनी सभा में बैठे थे, तो उन्हे खुद पर अभिमान हो आया।


वे सोचने लगे कि हम तीनों लोकों के स्वामी हैं। ब्राह्मण हमें यज्ञ में आहुति देते हैं और हमारी उपासना करते हैं। फिर हम सामान्य ब्राह्मण बृहस्पति से क्यों डरते हैं ?उनके आने पर क्यों खड़े हो जाते हैं?वे तो हमारी जीविका से पलते हैं। देवर्षि बृहस्पति देवताओं के गुरु थे।

अभिमान के कारण ऋषि बृहस्पति के पधारने पर न तो इन्द्र ही खड़े हुए और न ही अन्य देवों को खड़े होने दिया।देवगुरु बृहस्पति इन्द्र का ये कठोर दुर्व्यवहार देख कर चुप चाप वहां से लौट गए।कुछ देर पश्चात जब देवराज का मद उतरा तो उन्हे अपनी गलती का एहसास हुआ।
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@oyevivekk
THREAD:

80 Films with the biggest Plot Twists that will blow your mind.

1. Fight Club


2. Predestination


3. Seven


4. Requiem for a Dream
MOVIES , ALL , LIST OF MOVIES
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Sheetal Rijhwani
Sheetal Rijhwani
@RijhwaniSheetal
How you can filter stocks for Intraday trades - 🧵

As a kid, we would do homework before school the next day - you have to do homework here too. A specific sector performs on a particular day and studying things a day before will help you spot that particular sector. (1/11)

To prepare for the next trading day, you got to check the Index charts of all sectors. You got to keep an eye on a specific Index if you see a few factors:

- If any Index closed exactly at the resistance, you can get a trade on either side on the next day. (2/11)

- If it gave a rejection candle at resistance.

- If any Index closed exactly at support, you can get a trade on either side the following day.

- If there was a bullish signal at the support.

- If any Index gave a breakout or breakdown and then gave a good closing. (3/11)

- If any Index has taken support at 50 or 200 EMA.

Once you spot the Index, look at all the stocks in that particular Index. Pick at least 3-4 stocks from that Index and add them to your watchlist for the next day. It's ideal to check both higher and lower timeframes. (4/11)

What you should do next day? (When I am unable to check Indices and stocks a day before, I sometimes directly start with this process.)

- Start with checking the top gainers/losers list. I observe market from 9:15 to 9:45am to check the sectors performing well on that day.(5/11)
SCREENERS , ALL , INTRADAY STRATEGIES
Dr. Jane Clare Jones
Dr. Jane Clare Jones
@janeclarejones
So, on the subject of bonkers hyperbolic pretzeling over the Bell judgement, Grace 'destroy books I don't like & make inappropriate jokes about sterilising teenage girls' Lavery has some thoughts.

Tell me why my feminism is wrong Grace.

Oh

Well, if anyone thought the Bell judgment was going to make TRAs reconsider making massively overblown claims with no evidence backed up with nothing but a thick wadge of emotional blackmail.... HAHAHA, no one thought that.


A high court in the UK made a delimited judgment about teenager's ability to consent to puberty blockers. This puts all trans people everywhere in the world at risk.

Because if any human anywhere has any thoughts that deviate in any way from the rote line dictated by

the trans rights movement, this puts all trans people everywhere in mortal danger.

Let's be honest Grace. It doesn't put trans people at risk. It puts trans ideology at risk. Because trans ideology depends on the idea of innate gender identity, and the trans child is the

necessary material evidence of the ontology of gender identity.

That is, children are being medicalised to provide evidence to underwrite adults identities.

Nothing to see here.
LAW
\U0001f1fa\U0001f1f8I Support Our Military & Law Enforcement\U0001f1fa\U0001f1f8
🇺🇸I Support Our Milita...
@TheWiseOwl_AZ
1. There are more people added on the list of arrests and executions of famous people but no further intel is available at this time.

ARRESTS and EXECUTIONS OF FAMOUS PEOPLE 2020 UPDATED
Explanation of the latest military tribunal arrests and executions of famous people in 2020.

2. The tribunals are arresting these people in plain clothes. We saw the Obama’s arrest, although we didn’t know it was an arrest at the time. The arrest was done nonchalantly so as to not get noticed by the public.
All arrests of these famous people were done this way.

3. If the person is convicted of child sex trafficking, crimes against humanity or treason, they will be executed.
If the person arrested has evidence on someone else to help their prosecution, or is willing to help Trump, & they want to cooperate, they may be allowed to do so.

4. A sentence of execution down to life in prison may be accepted if their evidence is worth anything.
When a plea deal is done, the defendant must present evidence they have to make their sentence lighter. This information is given to the prosecution, defense, and to the judge.

5. If evidence is accepted, they are presented the plea deal. They will have to make a guilty plea to the crime with a video confession & written statement. That is how all plea deals work.

All famous people arrested were either treasonous or involved in satanic worship rituals.
CRIME