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Saved by @Daoyu15

https://t.co/hXlo8qgkD0
Look like that they got a classical case of PCR Cross-Contamination.
They had 2 fabricated samples (SRX9714436 and SRX9714921) on the same PCR run. Alongside with Lung07. They did not perform metagenomic sequencing on the “feces” and they did not get

 A positive oral or anal swab from anywhere in their sampling. Feces came from anus and if these were positive the anal swabs must also be positive. Clearly it got there after the NA have been extracted and were from the very low-level degraded RNA which were mutagenized from
The Taq. https://t.co/yKXCgiT29w to see SRX9714921 and SRX9714436.
Human+Mouse in the positive SRA, human in both of them. Seeing human+mouse in identical proportions across 3 different sequencers (PRJNA573298, A22, SEX9714436) are pretty straight indication that the originals
Were already contaminated with Human and mouse from the very beginning, and that this contamination is due to dishonesty in the sample handling process which prescribe a spiking of samples in ACE2-HEK293T/A549, VERO E6 and Human lung xenograft mouse.
The “lineages” they claimed to have found aren’t mutational lineages at all—all the mutations they see on these sequences were unique to that specific sequence, and are the result of RNA degradation and from the Taq polymerase errors accumulated from the nested PCR process
Where the extremely low error-ridden cross-contaminating molecule were amplified with mutagenesis happening on each step—especially between the initial RT-PCR and sequencing PCR process, which cross contamination have happened. Notice that any extra Pro or Cys on the highly
Conserved N protein sequences are lethal to the virus—especially the extra Cys which will become a nucleophile and a Ribonuclease in the budded virion in an extracellular environment, degrading the genome and inactivating the virus immediately. These Cys will also cause the N to
Dimerize and consequently lose it’s ability to bind RNA or mediate virion assembly in the first place. While the Pro will break a conserved secondary structure in the C-terminal domain and prevent the N from folding correctly. These are not found anywhere else and are obviously
Sequencing errors. Especially since these samples came from cross-contamination without a corresponding positive oral or anal swab (they didn’t get a single swab that is positive).
M1, they cite as having “hemorrhage” claimed to be Coronavirus symptoms, are anything BUT coronavirus symptoms. https://t.co/3a8nqY6rOz “Open in new tab
X-ray tests revealed a large area of shadow in the left lung of pangolin 2-Lishui, suggestive of pneumonia” but
“we have not found coronaviruses in these pangolins by meta-transcriptomic and PCR methods.”. Notice the PLT- in the Pestivirus-infected pangolin samples. Pestivirus is seen in the SRA of M1, SRX7756769 (107267359 spots as in the Xiao et al article). The Pestivirus is seen
In all PRJNA573298 sample lungs, and it causes interstitial pneumonia and gives the same kind of CT scan and blood test result as in the 06/22 article. This sample also contained a locally circulating CCoV (Canine Coronavirus, often infects zoo/rescued solitary animals due to
It’s ubiquitous nature in most human-associated environments) strain with reads that weren’t rolling circles as the GD-1 reads and are mostly related to the Canine Coronavirus isolates circulating in dogs in GZ.
Coronavirus infections can not generate rolling circles as they does not encode RNA ligases, an enzyme that is necessary to create a true CircRNA. (What they claim to be CircRNA reads from CoV infections in other papers aren’t true CircRNA but are Partially duplicated RNA due to
The Coronavirus RdRp template-switching to a location before it’s current location. This RNA is still linear. However, the Rolling Circles corresponds to two hotspots of M13 primers seen in the amplicons for nsp3 and for the 3’-end of the genome. This imply that they were
Remnants of cDNA cloning, stemming from DNA that were circularized without a vector backbone.)
Canine Coronavirus and FIPV (alphacoronavirus 1) infections also generate very similar symptoms, and will result in positive cross-reactive NP in the immunofluorescence screening. Even the right GEO profile provide they remove it from the final SRA. Finally, the ACE2 expression
“Lowest in the lungs” claimed in this paper is at odd with the 06/22 paper claim that “ACE2 and TMPRSS2 is most highly expressed in the lungs”. This suggest that the GEO profile of the 06/22 article associated with A22/SRX8582289 have been fabricated or were derived from hACE2
Mouse. The hemorrhage seen in M1 is consistent with DYPV(Flavivirus/pestivirus related to BVDV) which is seen in the metagenome, while the pneumonia could be either be the result of DYPV-related Pestivirus, from the Sendai (murine respirovirus), from the Canine Coronavirus or
From any of the non-Coronavirus (Colitivirus or Murine Respirovirus) agents found in PRJNA573298.
M1 have Murine Respirovirus.
https://t.co/ZJnLSxwe5o
SeV is in the same family as human Parainfluenza virus. It causes bilateral ground-glass opacity.
https://t.co/NeY2ajwsDo
The blood tests—pestivirus once again fully explains all the blood changed observed. When a Parainfluenza virus is also present, the blood test become indistinguishable.
Another snRNA-seq say ACE2 and TMPRSS2 are barely exiressed in the lungs and are never coexpressed.
Weaker than the kidneys—this is again at odds with the SCAU claim that even negative pangolins have lung>>kidney for ACE2. Both publication say that Kidney is the highest ACE2 expressing organ in all pangolins where lung expression is negligible. The 06/22 article is the only
One that claim that all pangolins including negative samples, have lung ACE2 >> Kidney. This indicate that they have Spiked all of them with K12-hACE2 mouse as it is in disagreement of all previous or later pangolin transcriptomes.
“(Individual number: L08) as a positive control”—and guess what L08 actually is—Human AND Mice!
L08: Lung08.

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Daoyu
Daoyu
@Daoyu15
@franciscodeasis https://t.co/OuQaBRFPu7
Unfortunately the "This work includes the identification of viral sequences in bat samples, and has resulted in the isolation of three bat SARS-related coronaviruses that are now used as reagents to test therapeutics and vaccines." were BEFORE the


chimeric infectious clone grants were there.https://t.co/DAArwFkz6v is in 2017, Rs4231.
https://t.co/UgXygDjYbW is in 2016, RsSHC014 and RsWIV16.
https://t.co/krO69CsJ94 is in 2013, RsWIV1. notice that this is before the beginning of the project

starting in 2016. Also remember that they told about only 3 isolates/live viruses. RsSHC014 is a live infectious clone that is just as alive as those other "Isolates".

P.D. somehow is able to use funds that he have yet recieved yet, and send results and sequences from late 2019 back in time into 2015,2013 and 2016!

https://t.co/4wC7k1Lh54 Ref 3: Why ALL your pangolin samples were PCR negative? to avoid deep sequencing and accidentally reveal Paguma Larvata and Oryctolagus Cuniculus?
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Daoyu
Daoyu
@Daoyu15
@AngloScot2 @WisdomRebel https://t.co/iHKwUoNLJ5

https://t.co/sxm17Eu9hV

https://t.co/FtjifSGItc
PRRA is highly purified in the CaLu-3 cell line when FBS is added to inhibit trypsin. Stu lied about that in his defense on the FCS identity. https://t.co/GzM7uA6vup the Gallaher article contain glaring

@WisdomRebel Mistakes and is completely impossible as HKU9 + RaTG13 only lead to a frameshift inactivated S and not a furin cleaved S.
https://t.co/gmDXK9OluN
The serological test the WIV claimed to be negative in Shi’s addendum is the exact same test they claimed positive in 2012 in the phD

@WisdomRebel thesis, and Stu once again lied about it. https://t.co/Uiy8U9BTYp significant evidence in the form of contaminated SRA datasets suggest that the DEFUSE grant have been funded in China, as the full-length HKU4 is not a part of the 2016-2019 grant, but is found before the start

@WisdomRebel Date of the 2020-2024 grant of June 2020. The WIV also contained several HKU3-like Coronaviruses, particularly in mice in an 2017 GEO experiment, which is consistent with the published HKU3 and batified mice experiment in the DEFUSE document (evidence from an already conducted

@WisdomRebel Experiment). Either DEFUSE or fast-tracked form of the 2020-2024 grant in 2019 would have lead to full-length Sarbecovirus clones being used, leading to SARS-CoV-2 in the lab—they used HKU4 in stead of HKU5 for the MERS spike experiment indicating that backbone sequence distance
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Daoyu
Daoyu
@Daoyu15
@VanGennepD @_benjaminv @stgoldst @acritschristoph @jbloom_lab @StevenSalzberg1 While they are allowed to engage with U.S. scientists, all their Q&A are pre-trained, pre-rehersed and all their speech and demonstration are carefully pre-scripted. You can even hear the long pauses between Shi’s words in all her webinars and interviews, indicative of a script

@_benjaminv @stgoldst @acritschristoph @jbloom_lab @StevenSalzberg1 Being handed to her and then read. No word that are outside of the script or are bad to the CCP’s official narrative (they are carefully trained and rehearsed before doing any webinars, meetings or interviews to U.S. scientists) are allowed in these engagements—they are allowed

@_benjaminv @stgoldst @acritschristoph @jbloom_lab @StevenSalzberg1 To engage but are not free. If they disclose anything that hurts the CCP narrative they and their families are disappeared.
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Daoyu
Daoyu
@Daoyu15
@jbloom_lab It seems that it is a deeply cultured, but in deed ancestrally derived, sequence of SARS-CoV-2, that have many of the VERO cell specific mutations and deletions already in.

but it is not likely a clinical specimen from China because of the USA/WA-1 base which is not in Wuhan.

It had T22657C, T3346C, A21562C and G487T. all of which is in RaTg13 but not in WuHu-1. also T1963C and T22963C in BANAL-52. not all of the reads were H655Y and del I68-T76.

And it is actually clustered closely to the A/North America lineage, which shouldn't have appeared in a Chinese lab. also not there in Wuhan either.

also only one of the SRRs harbored T18060C it seemed.
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Daoyu
Daoyu
@Daoyu15
@franciscodeasis https://t.co/Sd9IslUCH5 a FCS need a FCS in the inoculum to exist. It can not arise de-novo as it will be destroyed instantly by the immune system.

https://t.co/UgXygDjYbW a fourth Sars-like CoV is live at the WIV. This fourth virus is an infectious clone, where engineering of the S1-S2 is used regularly as mean to generate a culturable virus in HAE cells. No VERO E6 here, and HeLa-hACE2 is the new VERO of the WIV

https://t.co/DtjyycKy1v
https://t.co/PG7LVnHfsy
Even with VERO E6, only half the time does passage lead to the loss of the FCS—smaller plaques need to be explicitly picked for that to be a certainty.

Marburg virus is a novel virus that escaped from the lab. https://t.co/OGQM6qV27l the only reason why it did not become a pandemic is due to it being too lethal to sustain asymptomatic transmission in humans.

The highest reported number of cases were in WuChang right on top of the old WIV headquarters, In contrast to the population density data of Wuhan—note that the place near the market had the highest population density in all of Wuhan, which make it the most optimal location for
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AKASH BHAT
AKASH BHAT
@AKASHBH95801446
EXPLANATION OF THE EXISTENCE OF DARK MATTER AND DARK ENERGY !

What Is Dark Energy ?

More is unknown than is known. We know how much dark energy there is because we know how it affects the universe's expansion. Other than that, it is a complete mystery.

But it is an important mystery. It turns out that roughly 68% of the universe is dark energy. Dark matter makes up about 27%. The rest - everything on Earth, everything ever observed with all of our instruments, all normal matter - adds up to less than 5% of the universe.

This diagram reveals changes in the rate of expansion since the universe's birth 15 billion years ago. The more shallow the curve, the faster the rate of expansion. The curve changes noticeably about 7.5 billion years ago, when objects in the universe began flying apart as


a faster rate. Astronomers theorize that the faster expansion rate is due to a mysterious, dark force that is pulling galaxies apart.

WHAT OUR SANATAN DHARMA SAYS IN THIS CONTEXT LET’S TAKE A LOOK

The verses of Taittiriya Aranyaka say that only one pada of the Lord

is this manifested universe and the remaining three padas are outside it.

According to the observations and realizations of ancient seers, nearly eighty percent of this universe is yet of unknown nature which remains inactive during the process of creation (rather, evolution).
SCIENCE
foone
foone
@Foone
I think I've permanently confused KiCAD
it's reminding me that I need to connect this capacitor to Nothing


attach this end to ground, this end to +5v, and this end to THE VOID

I accidentally made all the capacitors gigantic and they won't fit anymore

halp


gonna design an ISA card using some of these

it's gonna be tricky to fit in the case


that's from this
SCIENCE
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Nick Hudson \U0001f642
Nick Hudson 🙂
@NickHudsonCT
Here is another very enjoyable conversation, with @Pandata19’s scientific advisory board member, Dr Jay Bhattacharya. Key ideas in this thread.

We are making world-changing decisions on the basis of evidence that is not very good. Vast scientific evidence tells us that infection fatality rates are much lower than originally expected. A small fraction of people get severe illness. 2/10

The scientific community has been resistant to evidence not supporting the majoritarian view, preferring instead to gin up panic, focusing on the worst case for everything the virus does & the best case for everything lockdowns do, and ignoring the range of uncertainty. 3/10

Academe is a strange place now, with debate stifled. But there's a sense some are opening up to considering opposing views. This is key, since suppression of views stops knowledge from progressing—the end of science. Public health norms of unified messaging complicate this. 4/10

Why did we, from the start, assume we knew nothing about this virus, instead of assuming a reasonable prior? Low susceptibility was evident early on. Assuming any virus is new is hard to square with our deep-time co-evolution with viruses, & their slow evolution. 5/10
SCIENCE
Rebecca \U0001f983 "Stay the Fuck Home" \U0001f983 Watson
Rebecca 🦃 "Stay the Fu...
@rebeccawatson
So it turns out that an organization I thought was doing good work, the False Memory Syndrome Foundation (associated with Center for Inquiry, James Randi, and Martin Gardner) was actually caping for pedophiles. Uhhhh oops?


Since this, bizarrely, turned out to be one of my longest videos ever (??) here's a quick thread to sum it up for those of you like myself with short attention spans. 1/10

In the '90s the False Memory Syndrome Foundation was founded to call attention to the problem of adults suddenly "remembering" child abuse that never actually happened, often under hypnosis. Skeptics like James Randi & Martin Gardner joined their board. 2/10

A new article reveals that the FMSF was founded by parents who had been credibly and PRIVATELY accused of molestation by their now-adult daughter. They publicized the accusation, destroyed the daughter's reputation, and started the foundation. 3/10

The FMSF assumed any accused pedo who joined was innocent, saying "We are a good-looking bunch of people, graying hair, well dressed, healthy, smiling; just about every person who has attended is someone you would surely find interesting and want to count as a friend" 😬 4/10
SCIENCE
James Hay
James Hay
@jameshay218
Ct values can be used to estimate epidemic dynamics UPDATE! Ct values are expected to change depending on whether the epidemic is growing or declining, and we harness this to estimate the epidemic trajectory. Lots of cool new analyses and methods!

Highlights:
- Cts from symptom-based surveillance change over time, but the effect is weaker
- Methods to infer incidence using single cross-sections of Cts
- Unbiased by changing testing coverage
- Gaussian process (wiggly line) model for incidence tracking using Ct values
2/12

This work is *JOINTLY led* with @LeekShaffer and PI’d by @michaelmina_lab. Thank you also to the ever insightful @mlipsitch and to coauthors @SanjatKanjilal @gabriel_stacey and @nialljlennon. 3/12

Premise: times since infection depend on the epidemic trajectory. Distributions of randomly sampled viral loads proxy times since infection. With calibration, Ct values can estimate growth rate. We focus on qPCR in SARS-CoV-2, but the principle applies to any outbreak. 4/12

Result 1: viral loads are shifted higher (Cts lower) during epidemic growth and lower (Cts higher) during decline when individuals are sampled *based on the onset of symptoms*. We simulated linelist data under symptom-based surveillance and looked at TSI and Cts over time. 5/12
SCIENCE

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Anu Satheesh \U0001f1ee\U0001f1f3
Anu Satheesh 🇮🇳...
@AnuSatheesh5
Neyyattinkara Krishna Kshetram
#Thiruvananthapuram
#KeralaTemples 🚩

Neyyattinkara Krishna Kshetram is considered as the Guruvayur of Trivandrum. Thrikkayyil Venna (butter) is main offering to Neyyattinkara Unnikannan. Kshetram is supposed to be built by Marthanda Varma.


Sthala Puranam says that, Anizham Thirunal Marthanda Varma was surrounded by his enemies, Ettuveettil Pillamar, while he was near the place where Temple is situated now. The king prayed to Padmanabhaswamy to protect him.  At that time, a small boy was seen there and he


advised the king to hide himself inside the hollow trunk of a jack fruit tree. The king was surprised to find a small boy in the forest, but agreed to the advice
Thus he was saved from the attack. When the enemies left, king tried to know about the boy, but he couldn't.


He believed that it was krishna himself who saved his life. A temple was built here, which is the present Neyyattinkara Krishna Kshetram. The jack fruit tree where  the king hid himself is now known as Ammachi Plavu. Ashtami Rohini, Vishu are very famous here

Hare Krishna
🙏🕉🙏
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Vibhu Vashisth
Vibhu Vashisth
@VIBHU_Tweet
RISHI PINGALA:THE ANCIENT INDIAN SAGE DISCREDITED FOR HIS MAJOR CONTRIBUTIONS TO MATHEMATICS & PHI

Have you ever heard about,"The Golden Ratio"?
The Golden Ratio or Phi(Greek) is a mathematical ratio of nature. Mother Nature is the most exquisite artist in herself.


&Its this Phi she uses to create all the beauty in World.
If anyone uses this ratio correctly in their art,it can turn into a spell-binding piece of work.Such is the significance of'Phi'.But my thread isn't about it.Its about somethin called'Fibonacci Series'&who actually made it


Phi is related to Fibonacci series(0,1,1,2,3,5,8,13,21,34...)
&this series is responsible for everything dat is beautiful on this planet. Just try to calculate the ratio of any 2 successive numbers ÷ each of them by the number before it in above infinite series.

E.g. 3/2=1.5, 5/3=1.666, 8/5=1.6, 34/21=1.61904 and so on. Did you notice something? The ratio is coming almost same every time. This ratio is called 'Phi 'which is numerically represented as 1:1.618. This Golden Spiral is made in exact proportion of 1:1.618.


The thing I want to emphasize here is that we always find only those things appealing and attractive that have Phi. It's in our subconscious. Isn't it some revelation? We are the part of this Divine proportion of the God or the Mother Nature. Check these:
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Vibhu Vashisth
Vibhu Vashisth
@VIBHU_Tweet
MAHARAANI TARARANI: THE QUEEN OF MARATHAS

Rani Tarabai was the brave daughter-in-law of Chhatrapati Shivaji and one of the greatest medieval Monarchs India ever had.
She was born in 1675 to one of the bravest commander-in-chiefs of Shivaji's Army named Hambir Rao Mohite.


Tarabai was just 8yrs old when she was married to Shivaji's younger son, Rajaram. Mughals and Marathas were constantly at war with each other. Shivaji was crowned the ruler of the Independent Maratha Swarajya in 1674 which was established by him.


However,the great leader died in 1680&the Maratha Samrajya went into a complete&total chaos.Marathas saw some very tough times ahead. In 1689,when Raigad Fort fell to the Mughal Army of over 15000,Shivaji's first wife Saibai and his elder son Sambhaji were captured&put to death.


Sambhaji's wife and son Shahu were taken prisoners to the Mughal court. During the same battle, Shivaji's second son Rajaram(Tarabai's husband) , who was the heir to the throne now, managed to escape using a disguise. He made his way to the Gingee Fort in Tamil Nadu.


Mughal General Zulfikar Khan, however, followed them to Gingee laying siege to the Gingee Fort. From 1690-1698,This was the longest siege by a Mughal Army of any Fort which stands testimony to the military and diplomatic credentials of Rani Tarabai.
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Catch me if you can @Mitesh_Engr

Time for a🧵
Mitesh Sir's EXPIRY Option Selling 101:
• What to look for?
• Strike Selection & Ratios
• SL mgmt
• Avoiding freezes
• Monthy Expiry
• Event days
• How he would have traded last expiry?

In collaboration with @AdityaTodmal


Edward de Bono says intelligence is like a🚗 Automobile

“A powerful car may be driven badly. A less powerful car may be driven well. The skill of the car driver determines how the power of the car is used.”


What @Mitesh_Engr Sir looks at for Expiry Trading:

• Technicals of BNF
• Individual Heavyweight stocks of BNF
• Support/Resistance of BNF on weekly TF
• Also has Superb SL management
• When it comes to adjustments, he is always on toes


How to Select Strikes to Sell?

• You can't sell based on premium
• Follow only trend
• Price action is best to sell on expiry day
• Never wait for the options to go to zero


Using Ratios to Sell:

• If BNF is in a range then strangles
• If breakout/ breakdown from a range then directional
• Selling 70/30 or 80/20 ratios
• 2 examples on how he used ratios
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Anshul Pandey
Anshul Pandey
@Anshulspiritual
RISHI ASHTAVAKRA(DEVAL)

Those who are well versed in our ancient scriptures must definitely have come across the name of Ashtavakra Muni. Who was he and what is the story behind his body getting crooked from all sides.

Muni Asit was the son of Pracheta Rishi.


He was childless and this caused a lot of worry to him. But an Akashvani assured him that he should go to Shivji to get all his
worries nullified.

So he along with his wife go to Shivji and after paying him obeisance start singing Shivji's praises.

He describes Shiv as the one who is beyond any other thing, whose glory has no words to describe. After this the couple stands with folded hands in front of Rudradev. The strotras sung in praise of Shiv possess magical qualities even today.

Shiv gets satisfied by them soon and is ready to grant a boon.

Shiva told him that, he very well knew what the Muni desired. He then gives him the "Sansaar Vijay' mantra and asks him to keep reciting it. The Isht devi would come at an appropriate time and fulfill his wish.

Asit Muni kept reciting this mantra for hundred years. The Devi appeared and granted him the boon of a son as a Shivansh. A son was born and was named as Deval. He grew upto be a great scholar well versed and a handsome young man. Asit Muni got him married to Ratnamalavati.
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