https://t.co/Azhyl8pDsX (2/7)
This is a thread on statistics in science: 1/7 (via @LogicofScience)
Basic Statistics Part 1: The Law of Large Numbers https://t.co/wUH8eAAIak
#Science #Statistics
https://t.co/LetN6aEBRM (3/7)
https://t.co/OtVrL0nyIl
Logic is the basis of any scientific argument. This is a thread on rules of logic by @LogicofScience
— The Desi Science Page (@desisciencepage) June 29, 2020
The Rules of Logic Part 1: Why Logic Always Workshttps://t.co/NpxZ5diZoK
1/7
May be mark this thread too for reading later? One on logical fallacies: https://t.co/Z7S9kNsFoI
-End-
Let's talk about logical fallacies:
— The Desi Science Page (@desisciencepage) June 25, 2020
"Perhaps the most common mistake that people make in debates is the use of logical fallacies. This occurs largely because people generally are not taught logical fallacies, and, therefore, don\u2019t recognize them when they use or see them"
1/n pic.twitter.com/P7xVY9Ehln
More from Science
https://t.co/hXlo8qgkD0
Look like that they got a classical case of PCR Cross-Contamination.
They had 2 fabricated samples (SRX9714436 and SRX9714921) on the same PCR run. Alongside with Lung07. They did not perform metagenomic sequencing on the “feces” and they did not get
A positive oral or anal swab from anywhere in their sampling. Feces came from anus and if these were positive the anal swabs must also be positive. Clearly it got there after the NA have been extracted and were from the very low-level degraded RNA which were mutagenized from
The Taq. https://t.co/yKXCgiT29w to see SRX9714921 and SRX9714436.
Human+Mouse in the positive SRA, human in both of them. Seeing human+mouse in identical proportions across 3 different sequencers (PRJNA573298, A22, SEX9714436) are pretty straight indication that the originals
Were already contaminated with Human and mouse from the very beginning, and that this contamination is due to dishonesty in the sample handling process which prescribe a spiking of samples in ACE2-HEK293T/A549, VERO E6 and Human lung xenograft mouse.
The “lineages” they claimed to have found aren’t mutational lineages at all—all the mutations they see on these sequences were unique to that specific sequence, and are the result of RNA degradation and from the Taq polymerase errors accumulated from the nested PCR process
Look like that they got a classical case of PCR Cross-Contamination.
They had 2 fabricated samples (SRX9714436 and SRX9714921) on the same PCR run. Alongside with Lung07. They did not perform metagenomic sequencing on the “feces” and they did not get
A positive oral or anal swab from anywhere in their sampling. Feces came from anus and if these were positive the anal swabs must also be positive. Clearly it got there after the NA have been extracted and were from the very low-level degraded RNA which were mutagenized from
The Taq. https://t.co/yKXCgiT29w to see SRX9714921 and SRX9714436.
Human+Mouse in the positive SRA, human in both of them. Seeing human+mouse in identical proportions across 3 different sequencers (PRJNA573298, A22, SEX9714436) are pretty straight indication that the originals
Were already contaminated with Human and mouse from the very beginning, and that this contamination is due to dishonesty in the sample handling process which prescribe a spiking of samples in ACE2-HEK293T/A549, VERO E6 and Human lung xenograft mouse.
The “lineages” they claimed to have found aren’t mutational lineages at all—all the mutations they see on these sequences were unique to that specific sequence, and are the result of RNA degradation and from the Taq polymerase errors accumulated from the nested PCR process
Hard agree. And if this is useful, let me share something that often gets omitted (not by @kakape).
Variants always emerge, & are not good or bad, but expected. The challenge is figuring out which variants are bad, and that can't be done with sequence alone.
You can't just look at a sequence and say, "Aha! A mutation in spike. This must be more transmissible or can evade antibody neutralization." Sure, we can use computational models to try and predict the functional consequence of a given mutation, but models are often wrong.
The virus acquires mutations randomly every time it replicates. Many mutations don't change the virus at all. Others may change it in a way that have no consequences for human transmission or disease. But you can't tell just looking at sequence alone.
In order to determine the functional impact of a mutation, you need to actually do experiments. You can look at some effects in cell culture, but to address questions relating to transmission or disease, you have to use animal models.
The reason people were concerned initially about B.1.1.7 is because of epidemiological evidence showing that it rapidly became dominant in one area. More rapidly that could be explained unless it had some kind of advantage that allowed it to outcompete other circulating variants.
Variants always emerge, & are not good or bad, but expected. The challenge is figuring out which variants are bad, and that can't be done with sequence alone.
Feels like the next thing we're going to need is a ranking system for how concerning "variants of concern\u201d actually are.
— Kai Kupferschmidt (@kakape) January 15, 2021
A lot of constellations of mutations are concerning, but people are lumping together variants with vastly different levels of evidence that we need to worry.
You can't just look at a sequence and say, "Aha! A mutation in spike. This must be more transmissible or can evade antibody neutralization." Sure, we can use computational models to try and predict the functional consequence of a given mutation, but models are often wrong.
The virus acquires mutations randomly every time it replicates. Many mutations don't change the virus at all. Others may change it in a way that have no consequences for human transmission or disease. But you can't tell just looking at sequence alone.
In order to determine the functional impact of a mutation, you need to actually do experiments. You can look at some effects in cell culture, but to address questions relating to transmission or disease, you have to use animal models.
The reason people were concerned initially about B.1.1.7 is because of epidemiological evidence showing that it rapidly became dominant in one area. More rapidly that could be explained unless it had some kind of advantage that allowed it to outcompete other circulating variants.
Epic thread incoming:
I'm going to answer the question so many people have been asking this week:
WHAT IS PROJECT X???
Here's the definitive thread to tell you - and show you -precisely what Project X is
Grab a drink, sit down with me and let's #TalkLiberation
<3
1/?
"Project X" is actually called "PanQuake".
Pan means "all". Quake is the huge effect our voices can have when our communications are uncensored and when we have access to brand new functionality that *enhances* our social reach, rather than diminishes it
Here's our logo:
2/?
You can follow the fledgling official PanQuake Twitter account here: @pan_quake and see our super cool new website here: https://t.co/F7wLSeM6aK
You can find our donation page here: https://t.co/VICFnsR0RX
Keep reading this thread to find out why we created it & what it is
3/?
SPOILER ALERT: Much of the content below this point is from my personal slides & speech notes from today's launch event. That stream got totally ruined by (big) tech problems, but I'm happy to report everything is turning out wonderfully
Here are some of our most high profile & dedicated public advocates for PanQuake - many of whom were scheduled to appear at our launch. All of whom stuck around for hours, to do a prerecord of the event, which is being edited, processed & uploaded for you as I write this.
5/?
I'm going to answer the question so many people have been asking this week:
WHAT IS PROJECT X???
Here's the definitive thread to tell you - and show you -precisely what Project X is
Grab a drink, sit down with me and let's #TalkLiberation
<3
1/?
"Project X" is actually called "PanQuake".
Pan means "all". Quake is the huge effect our voices can have when our communications are uncensored and when we have access to brand new functionality that *enhances* our social reach, rather than diminishes it
Here's our logo:
2/?
You can follow the fledgling official PanQuake Twitter account here: @pan_quake and see our super cool new website here: https://t.co/F7wLSeM6aK
You can find our donation page here: https://t.co/VICFnsR0RX
Keep reading this thread to find out why we created it & what it is
3/?
SPOILER ALERT: Much of the content below this point is from my personal slides & speech notes from today's launch event. That stream got totally ruined by (big) tech problems, but I'm happy to report everything is turning out wonderfully
Not one single team member or guest left. We are all still here, smiling not crying, as we record this event and will get it out to you all very soon :)
— Suzie Dawson (@Suzi3D) January 17, 2021
I'm so proud of everyone, what an amazing crewhttps://t.co/RmE0BicIXF
Here are some of our most high profile & dedicated public advocates for PanQuake - many of whom were scheduled to appear at our launch. All of whom stuck around for hours, to do a prerecord of the event, which is being edited, processed & uploaded for you as I write this.
5/?
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Speech Delay is most common in children nowadays
In ancient times, our grandparents used to follow typical natural way of caring the needs of a child. All they used were more of natural products than chemical based for the growth of child.
One of major step followed was to feed Gurbach Jadd/ Vasa Kommu/ Acorus Calamus for initiating good speech ability in a child. This stem was needed to babies on Tuesdays and Sundays in mother's milk.
Vasa is feed to baby after the 1st bath on 12th day in week. Weekly only thrice it is fed and named as :
Budhwar - Budhi Vasa
Mangalwar - Vaak Vasa
Ravi Vaar - Aayush Vasa
This stem is burnt and rubbed against the grinding stone in mother's milk or warm water to get a paste
The procedure to make it is in the link
https://t.co/uo4sGp7mUm
It should not be given daily to the child. Other main benefits are
1. It clears the phlegm in child's throat caused due to continuous milk intake. It clears the tracts and breathing is effortless.
2. Digestion
For children who haven't got their speech and is delayed than usual should feed this vasa on these days in week atleast for 6months. Don't get carried away with this dialogue
"Some gain speech little late"
In ancient times, our grandparents used to follow typical natural way of caring the needs of a child. All they used were more of natural products than chemical based for the growth of child.
One of major step followed was to feed Gurbach Jadd/ Vasa Kommu/ Acorus Calamus for initiating good speech ability in a child. This stem was needed to babies on Tuesdays and Sundays in mother's milk.
Vasa is feed to baby after the 1st bath on 12th day in week. Weekly only thrice it is fed and named as :
Budhwar - Budhi Vasa
Mangalwar - Vaak Vasa
Ravi Vaar - Aayush Vasa
This stem is burnt and rubbed against the grinding stone in mother's milk or warm water to get a paste
The procedure to make it is in the link
https://t.co/uo4sGp7mUm
It should not be given daily to the child. Other main benefits are
1. It clears the phlegm in child's throat caused due to continuous milk intake. It clears the tracts and breathing is effortless.
2. Digestion
For children who haven't got their speech and is delayed than usual should feed this vasa on these days in week atleast for 6months. Don't get carried away with this dialogue
"Some gain speech little late"
