He was complaining about how he had hired his video projector out for a party, and it had come back with one of the lenses missing.
@I_Make_It_All One approach that i have found that works, is to work out how you can make a new tool pay for itself.
After that point, it's yours, free and clear. :)
He was complaining about how he had hired his video projector out for a party, and it had come back with one of the lenses missing.
This was his original reason for buying the machine.
He hadn't thought about it, so he started scribbling down some figures.
He didn't NEED to hire it out again.
He could afford to say No to annoying customers. :D
I'll find ways of making the hardware pay for itself, then after that it's mine to use as i wish. :D
Everything after that is a bonus. :D
Every machine i work on, should be able to pay for itself, and as long as that the time taken to do that, is a fraction of the machine's rated lifespan, then it's a profitable purchase. :D
It'll pay for itself in 24-36 months, but with maintenance, it'll last for 50 years. :D
Once the first 3 years are done, it's free for the next 47 years. :D
How long will it last?
How much does it cost?
How long till i make my original money back?
After that i don't need to hire it out. :D
More from Science
https://t.co/hXlo8qgkD0
Look like that they got a classical case of PCR Cross-Contamination.
They had 2 fabricated samples (SRX9714436 and SRX9714921) on the same PCR run. Alongside with Lung07. They did not perform metagenomic sequencing on the “feces” and they did not get
A positive oral or anal swab from anywhere in their sampling. Feces came from anus and if these were positive the anal swabs must also be positive. Clearly it got there after the NA have been extracted and were from the very low-level degraded RNA which were mutagenized from
The Taq. https://t.co/yKXCgiT29w to see SRX9714921 and SRX9714436.
Human+Mouse in the positive SRA, human in both of them. Seeing human+mouse in identical proportions across 3 different sequencers (PRJNA573298, A22, SEX9714436) are pretty straight indication that the originals
Were already contaminated with Human and mouse from the very beginning, and that this contamination is due to dishonesty in the sample handling process which prescribe a spiking of samples in ACE2-HEK293T/A549, VERO E6 and Human lung xenograft mouse.
The “lineages” they claimed to have found aren’t mutational lineages at all—all the mutations they see on these sequences were unique to that specific sequence, and are the result of RNA degradation and from the Taq polymerase errors accumulated from the nested PCR process
Look like that they got a classical case of PCR Cross-Contamination.
They had 2 fabricated samples (SRX9714436 and SRX9714921) on the same PCR run. Alongside with Lung07. They did not perform metagenomic sequencing on the “feces” and they did not get
A positive oral or anal swab from anywhere in their sampling. Feces came from anus and if these were positive the anal swabs must also be positive. Clearly it got there after the NA have been extracted and were from the very low-level degraded RNA which were mutagenized from
The Taq. https://t.co/yKXCgiT29w to see SRX9714921 and SRX9714436.
Human+Mouse in the positive SRA, human in both of them. Seeing human+mouse in identical proportions across 3 different sequencers (PRJNA573298, A22, SEX9714436) are pretty straight indication that the originals
Were already contaminated with Human and mouse from the very beginning, and that this contamination is due to dishonesty in the sample handling process which prescribe a spiking of samples in ACE2-HEK293T/A549, VERO E6 and Human lung xenograft mouse.
The “lineages” they claimed to have found aren’t mutational lineages at all—all the mutations they see on these sequences were unique to that specific sequence, and are the result of RNA degradation and from the Taq polymerase errors accumulated from the nested PCR process
It's another stunning Malagasy #dartfrog/#poisonfrog for today's #FrogOfTheDay, #42 Mantella cowani Boulenger, 1882! A highly threatened, actively conserved and managed frog from the highlands of central #Madagascar
#MadagascarFrogs
📸D.Edmonds/CalPhotos
This thread will cover only a tiny fraction of the work on Mantella cowanii because, being so charismatic and threatened, it has received quite a bit of attention.
#MadagascarFrogs
We start at the very beginning: the first specimens, two females, were collected by Reverend Deans Cowan in East Betsileo, Madagascar, and sent to London, where George Albert Boulenger described the species in 1882.
#MadagascarFrogs
Boulenger placed the species in his new genus, Mantella, along with ebenaui, betsileo, and madagascariensis. He recognised that the other Malagasy poison frogs were distinct from the Dendrobates of the Americas, although he did keep them in the Dendrobatidae.
#MadagascarFrogs
As more specimens were collected, it became clear that the species was highly variable. In 1978, Jean Guibé wrote with interest about this variability, describing a new subspecies, M. cowani nigricans—today a full species. #MadagascarFrogs
https://t.co/dwaHMbrYbj
#MadagascarFrogs
📸D.Edmonds/CalPhotos
This thread will cover only a tiny fraction of the work on Mantella cowanii because, being so charismatic and threatened, it has received quite a bit of attention.
#MadagascarFrogs
We start at the very beginning: the first specimens, two females, were collected by Reverend Deans Cowan in East Betsileo, Madagascar, and sent to London, where George Albert Boulenger described the species in 1882.
#MadagascarFrogs
Boulenger placed the species in his new genus, Mantella, along with ebenaui, betsileo, and madagascariensis. He recognised that the other Malagasy poison frogs were distinct from the Dendrobates of the Americas, although he did keep them in the Dendrobatidae.
#MadagascarFrogs
As more specimens were collected, it became clear that the species was highly variable. In 1978, Jean Guibé wrote with interest about this variability, describing a new subspecies, M. cowani nigricans—today a full species. #MadagascarFrogs
https://t.co/dwaHMbrYbj
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THREAD PART 1.
On Sunday 21st June, 14 year old Noah Donohoe left his home to meet his friends at Cave Hill Belfast to study for school. #RememberMyNoah💙
He was on his black Apollo mountain bike, fully dressed, wearing a helmet and carrying a backpack containing his laptop and 2 books with his name on them. He also had his mobile phone with him.
On the 27th of June. Noah's naked body was sadly discovered 950m inside a storm drain, between access points. This storm drain was accessible through an area completely unfamiliar to him, behind houses at Northwood Road. https://t.co/bpz3Rmc0wq
"Noah's body was found by specially trained police officers between two drain access points within a section of the tunnel running under the Translink access road," said Mr McCrisken."
Noah's bike was also found near a house, behind a car, in the same area. It had been there for more than 24 hours before a member of public who lived in the street said she read reports of a missing child and checked the bike and phoned the police.
On Sunday 21st June, 14 year old Noah Donohoe left his home to meet his friends at Cave Hill Belfast to study for school. #RememberMyNoah💙
He was on his black Apollo mountain bike, fully dressed, wearing a helmet and carrying a backpack containing his laptop and 2 books with his name on them. He also had his mobile phone with him.
On the 27th of June. Noah's naked body was sadly discovered 950m inside a storm drain, between access points. This storm drain was accessible through an area completely unfamiliar to him, behind houses at Northwood Road. https://t.co/bpz3Rmc0wq
"Noah's body was found by specially trained police officers between two drain access points within a section of the tunnel running under the Translink access road," said Mr McCrisken."
Noah's bike was also found near a house, behind a car, in the same area. It had been there for more than 24 hours before a member of public who lived in the street said she read reports of a missing child and checked the bike and phoned the police.
