Check out our last preprint- We use single-cell & spatial technologies to map the human uterus and inform organoids experiments

Mapping the temporal and spatial dynamics of the human endometrium in vivo and in vitro

We generate single-cell and spatial reference maps of the human uterus and dissect the signalling pathways that determine cell fate of the epithelial lineages in the lumenal and glandular microenvironments.
To study cell-signalling we use Cell2location (@vitaliikl) and develop CellPhoneDBv3 that considers cellular coordinates for the inference of cell-cell communication
We mapped 3D endometrial organoid cultures and benchmark the model to our uterine atlas. To do so, we develop a novel computational pipeline to align in vitro and in vivo datasets.
Our comparison highlights common pathways regulating the differentiation of secretory and ciliated lineage in vivo and in vitro.
Informed by the in vivo dataset, we show in vitro that downregulation of WNT or NOTCH pathways increases the differentiation efficiency along the secretory and ciliated lineages, respectively
We are excited about how our mechanistic insights of the healthy endometrium may help us understand neglected uterine pathologies, such as endometriosis or endometrial cancer
Amazing collaborative work with Turco lab @CamPathology @teichlab @bayraktar_lab led by amazing scientists @LuzGarAl @krobertssci @NikolKons & Louis-F Hanfield
A big thank you @MoffettAm485 for all fruitful discussions & help. Such an amazing journey!
Big thanks also @luiza_moore @KSaebParsy & many others for your guidance, discussions and sample acquisition.

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Ok, I’ve told this story a few times, but maybe never here. Here we go. 🧵👇


I was about 6. I was in the car with my mother. We were driving a few hours from home to go to Orlando. My parents were letting me audition for a tv show. It would end up being my first job. I was very excited. But, in the meantime we drove and listened to Rush’s show.

There was some sort of trivia question they posed to the audience. I don’t remember what the riddle was, but I remember I knew the answer right away. It was phrased in this way that was somehow just simpler to see from a kid’s perspective. The answer was CAROUSEL. I was elated.

My mother was THRILLED. She insisted that we call Into the show using her “for emergencies only” giant cell phone. It was this phone:


I called in. The phone rang for a while, but someone answered. It was an impatient-sounding dude. The screener. I said I had the trivia answer. He wasn’t charmed, I could hear him rolling his eyes. He asked me what it was. I told him. “Please hold.”

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One of the most successful stock trader with special focus on cash stocks and who has a very creative mind to look out for opportunities in dark times

Covering one of the most unique set ups: Extended moves & Reversal plays

Time for a 🧵 to learn the above from @iManasArora

What qualifies for an extended move?

30-40% move in just 5-6 days is one example of extended move

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10WMA =


Another hack to identify extended move in a stock:

Too many green days!

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