BC SCIENCE
Saved by @CodyyyGardner

Let Uncle Ralph educate you
Synthetic Viral Genomics:
Risks and Benefits for Science and Society

 "Alternatively, “No See’m” sites can be used to insert foreign genes into viral, eukaryotic, or microbial genome or vector, simultaneously removing all evidence of the restriction sites that were used in the recombinant DNA manipulation"
By orientating the restriction sites as “No See’m”, the sites are removed during reassembly, leaving only the desired mutation in the final DNA product.
The dual properties of strand specificity & a variable end overhang that can be tailored to match any sequence allow for Esp3I sites to be engineered as “universal connectors” that can be joined with any other four nucleotide restriction site overhangs (e.g. EcoRI, PstX1, BamH1)
Seamless assembly (also called No See’m Sites (85)) cascades have been used to assemble full length cDNAs of the coronaviruses mouse hepatitis virus, transmissible gastroenteritis virus, infectious bronchitis virus and SARS-CoV (Refs: 85,86,87)
Type IIS restriction endonucleases recognize asymmetric binding sites & leave asymmetric ends
These enzymes can be used to create unique interconnecting junctions, which can be subsequently removed from final assembly product allowing seamless reconstruction of an exact sequence
With enzymes like Esp3I, interconnecting restriction site junctions can be located at ends of each cDNA & systematically removed during assembly of complete full-length cDNA product
Consequently, knowledgeable experts can theoretically reconstruct full length synthetic genomes for any of the high priority virus pathogens, although technical concerns may limit the robustness of these approaches.
Another approach might be to “humanize” zoonotic viruses by inserting mutations into virus attachment proteins or constructing chimeric proteins that regulate virus species specificity (viral attachment proteins bind receptors, mediating virus docking and entry into cells).
The pathogenicity of these chimeric coronaviruses is unknown
The pathogenicity of these chimeric coronaviruses is unknown
The pathogenicity of these chimeric coronaviruses is unknown
The pathogenicity of these chimeric coronaviruses is unknown
"Scapegoat option"
This powerful technique provides bioterrorists with a “scapegoat” option; leaving a sequence signature that misdirects efforts at tracking the true originators of the crime. Even better, the approach could be used to build mistrust &/or precipitate open warfare
Dual Use
Uncle Ralph summarises his findings (1)
Uncle Ralph Concludes (2)
Hence Ecohealth DARPA/DTRA spooks & virus thieves collaboration with Baric (UNC) Lipkin (Mailman) NIchols (Atlanta CDC) and USAMRIID (Bavari, Totura et al) & Jonathan Epstein's palpable concern about dual use references in the @USRightToKnow FOIA emails
A scary read for an experimental monkey!
Delving into the cold and calculating mind of a twisted genius?
https://t.co/MrwxYK93cx
Bring Uncle Ralph and his transgenic mice in for questioning!
unroll @threadreaderapp
You just read the Baric Paper, one of 6 in a series, the rest are available here in a 191 page document
https://t.co/rjNZ8aDNDl
Related Papers
1. Synthetic Genomics: Options for Governance (2008) https://t.co/A32jEfL1TQ
2. Sequence Screening - Robert Jones (2005)
https://t.co/zOqMroYbVU
3. Synthetic Biology as a Field of Dual-Use Bioethical Concern - Alexander Kelle
https://t.co/Va1movPqvs
Related papers (2)
4. Sanghvi Y. A Roadmap to the Assembly of Synthetic DNA from Raw Materials.
https://t.co/4kWu4vzcXC
5. Collett MS. Impact of Synthetic Genomics on the Threat of Bioterrorism with Viral Agents.
https://t.co/OcaTmuCUBy
Related Papers (3)
6. Fleming DO. Risk Assessment of Synthetic Genomics: A Biosafety & Biosecurity Perspective.
https://t.co/JBcLVLr005
7. Risk Governance of Synthetic Biology
https://t.co/9BE9X5s0Fb
8. US Competitiveness in Synthetic Biology
https://t.co/OCZJdsXSjl
Related papers (4)
9. Ensuring security of synthetic biology
https://t.co/7kPE1OpbrS
10. Synthetic biology: emerging research field in China
https://t.co/T3BcaDvUsa
11.
What rough beast? Synthetic biology, uncertainty,& the future of biosecurity (2016)
https://t.co/XTlBXILEWh
Back to Baric et Al
Reverse genetics with a full-length infectious cDNA of severe acute respiratory syndrome coronavirus (2003)
https://t.co/k8PI1rbcm8
Systematic Assembly of a Full-Length Infectious Clone of Human Coronavirus NL63 (2008)
https://t.co/bWhAv1wsC0
For the Record (WIV)
In 2016, Shi and her team at the WIV, in conjunction with the New York-based EcoHealth Alliance, constructed a full-length clone of a bat coronavirus called SL-CoV WIV1. They assembled it in discrete segments.
They genetically engineered the virus using the pGEM®-T Easy Vector Systems to join the segments. This system, also available on the internet, gives researchers several options for how to remove GM inserts that can be seen as signatures of a lab-made virus.18
pGEM®-T Easy Vector Systems:
“Thus, several options exist to remove the desired insert DNA with a single restriction digestion.”
This shows that researchers at the WIV have the ability to genetically engineer viruses and remove the signatures of the genetic engineering.
WIV & EcoHealth Alliance published a paper in 2017 on how they genetically modified spike proteins of 8 bat coronaviruses, by cutting & pasting genetic material from other coronaviruses, so that the viruses infected the human ACE2 receptor
They used pGEM®-T Easy Vector Systems to join the segments to genetically engineer these viruses.
They showed how they can insert new spikes into viruses. The researchers state:
“Then any spike could be substituted into the genome of SARSr-CoV WIV1 through this strategy.”
This shows that researchers at WIV have the ability to genetically modify multiple coronaviruses to insert new spikes, and these new viruses cannot be detected as genetically engineered.
The research clearly shows that GOF researchers at WIV could assemble SARS-CoV-2 from bat coronaviruses, such as RaTG13 or similar & spike protein from Malayan Pangolins & insert multi-basic cleavage sites into precise regions of spike & leave no evidence of genetic engineering.
All the above about WIV research is taken verbatim from an excellent article by Andre Leu on the website of @OrganicConsumer
COVID 19: The Spike and the Furin Cleavage
https://t.co/bDOMmhpJ1t

More from Billy Bostickson 🏴👁&👁 🆓

Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193
Billy Bostickson 🏴👁&👁 ...
@BillyBostickson
Keep dwelling on this:
Further Examination of the Motif near PRRA Reveals Close Structural Similarity to the SEB Superantigen as well as Sequence Similarities to Neurotoxins and a Viral SAg.

The insertion PRRA together with 7 sequentially preceding residues & succeeding R685 (conserved in β-CoVs) form a motif, Y674QTQTNSPRRAR685, homologous to those of neurotoxins from Ophiophagus (cobra) and Bungarus genera, as well as neurotoxin-like regions from three RABV strains

(20) (Fig. 2D). We further noticed that the same segment bears close similarity to the HIV-1 glycoprotein gp120 SAg motif F164 to V174.
https://t.co/EwwJOSa8RK


In (B), the segment S680PPRAR685 including the PRRA insert and highly conserved cleavage site *R685* is shown in van der Waals representation (black labels) and nearby CDR residues of the TCRVβ domain are labeled in blue/white
https://t.co/BsY8BAIzDa


Sequence Identity %
https://t.co/BsY8BAIzDa
Y674 - QTQTNSPRRA - R685
Similar to neurotoxins from Ophiophagus (cobra) & Bungarus genera & neurotoxin-like regions from three RABV strains
T678 - NSPRRA- R685
Superantigenic core, consistently aligned against bacterial or viral SAgs
ALL
Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193
Billy Bostickson 🏴👁&👁 ...
@BillyBostickson
1/x Fort Detrick History
Mr. Patrick, one of the chief scientists at the Army Biological Warfare Laboratories at Fort Detrick in Frederick, Md., held five classified US patents for the process of weaponizing anthrax.

2/x
Under Mr. Patrick’s direction, scientists at Fort Detrick developed a tularemia agent that, if disseminated by airplane, could cause casualties & sickness over 1000s mi². In a 10,000 mi² range, it had 90% casualty rate & 50% fatality rate


3/x His team explored Q fever, plague, & Venezuelan equine encephalitis, testing more than 20 anthrax strains to discern most lethal variety. Fort Detrick scientists used aerosol spray systems inside fountain pens, walking sticks, light bulbs, & even in 1953 Mercury exhaust pipes


4/x After retiring in 1986, Mr. Patrick remained one of the world’s foremost specialists on biological warfare & was a consultant to the CIA, FBI, & US military. He debriefed Soviet defector Ken Alibek, the deputy chief of the Soviet biowarfare program
https://t.co/sHqSaTSqtB


5/x Back in Time
In 1949 the Army created a small team of chemists at "Camp Detrick" called Special Operations Division. Its assignment was to find military uses for toxic bacteria. The coercive use of toxins was a new field, which fascinated Allen Dulles, later head of the CIA
ALL
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Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193
Billy Bostickson 🏴👁&👁 ...
@BillyBostickson
1. Updated Thread on:
A, Wuhan Seafood Market Positive Environmental Samples, B. Early Local Reports about Animals at the Market
C. CCP Censorship and Police harassment of local activists uploading censored news reports to GitHub.
The original thread was a mess!

2. CCP Censorship & Police harassment of local activists uploading censored news reports to GitHub.
It all started with a simple search query:
"market" "animal" on a github database:
https://t.co/q2HxuUuHKa
Only to find that it had been deleted on github due to CCP pressure!

3. Github is Censorship proof, right?
It does not get deleted! But..
https://t.co/k7BJWUgWDq
Arms get Twisted!
https://t.co/ckzicj7NlE
Please remember these brave young activists for creating a permanent record of censored news from the early days while CCP rewrites history


4. What a shock!
But thanks to the very nature of GitHub, "forks" and "clones" had been made & saved.
The best one is here:
https://t.co/ZWiyWK9SAa
Obviously all in Chinese ;) but tks to Google Chrome we can right click translate to English & find archived scrubbed Chinese news


5. "This is an archive of in-depth reports and personal narratives on the COVID-19 outbreak in China"
Contains media reports, non-fictional works and personal narratives related to the 2020 coronavirus pneumonia.
Part of the archive is provided by netizens, I am grateful.
ALL
Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193
Billy Bostickson 🏴👁&👁 ...
@BillyBostickson
Meet Yang Ruifu, CCP's biological weapons expert https://t.co/JjB9TLEO95 via @Gnews202064
Interesting expose of China's top bioweapons expert who oversaw fake pangolin research
Paper 1: https://t.co/TrXESKLYmJ
Paper 2:
https://t.co/9LSJTNCn3l
Pangolin
https://t.co/2FUAzWyOcv


Ruifu, obviously obsessed with PLAGUE,
https://t.co/7A9NiRlNoz
is a friend of Oliver Pybus (one of the Holmes, Andersen, Rambaut, Lipkin Gang)
Oliver Pybus helped Ruifu pump out CCP Propaganda
see next tweet

Pybus and Ruifu
An investigation of transmission control measures during the first 50 days of the COVID-19 epidemic in China
https://t.co/fl8yh0PSI8
and
China's control measures may have prevented 700,000 COVID-19

Email for academic queries:
[email protected] (H.T.);
[email protected] (C.D.); [email protected] (O.G.P.);
[email protected] (B.T.G.)
[email protected] (R.Y.)


Pangoscam
ALL
Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193
Billy Bostickson 🏴👁&👁 ...
@BillyBostickson
Digital Operations Group (DOG) 77th Brigade
A pathetic digestive biscuit munching bunch of British Berks from civvy street whose badge dishonours the Chindits.
Sleazy Media Manipulators who usually fail to deliver "influencing activity & products" to counter narratives of others


“If everybody is thinking alike then somebody isn’t thinking”
Just about sums up their Covid-19 "narrative"
“Behavioural change is our USP [unique selling point]”
They fail every time, doubt they could even sell their asses to soldiers at Denison Barracks

More on this pathetic bunch of Trolls scrounging a living from honest taxpayers' money to sell offal scraped off the Butcher's

Why are these little British trolls all over Twitter lately?
The have been ordered to counter "Covid- 19 Myths"
One of these is Myth No.3 (see image)
2004-Factsheet-Russia-Myths-COVID-19_en.pdf
https://t.co/PxjOPJjWH0


🐇🕳️💩
https://t.co/r6n1t1wYvG
https://t.co/aLuzKktcZv
https://t.co/zFmaD9yadQ
https://t.co/UkSdag9U5H
https://t.co/3N3XwF9Drj
https://t.co/ZyKXL2uLzB
https://t.co/OIlpoMVMcA
https://t.co/e0W3qWkwvL
https://t.co/NMdiTqtU4u
https://t.co/gzYiVWeTJf
ALL

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Katie Mack
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Light travels at about 1 foot per nanosecond.

Hold your hand up 12 inches from your face: you’re seeing your hand as it was a nanosecond ago. Everything you look at is, to one degree or another, in the past. The farther away in space, the more ancient in time.

You can’t see the Sun as it is now, but you can see it as it was about 8 minutes ago. You can’t see Alpha Centauri now, but you can see it 4.4 years ago. You can see the Andromeda Galaxy as it was 2.5 million years in the past. And so on.

With powerful telescopes, we can see galaxies whose light has been traveling to us for more than 13 billion years. We see them shining in a universe that’s still young, where gravity has just begun to pull matter together into stars and galaxies.

We can see something even more distant, and more ancient, than the first galaxies. If we peer out far enough, in between the galaxies, we can see parts of the Universe that are so far away, it has taken the light from that distance almost the entire age of the cosmos to reach us.
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Eric Feigl-Ding
Eric Feigl-Ding
@DrEricDing
JUST ONE PERSON—UK 🇬🇧 scientists think one immunocompromised person who cleared virus slowly & only partially wiped out an infection, leaving behind genetically-hardier viruses that rebound & learn how to survive better. That’s likely how #B117 started. 🧵 https://t.co/bMMjM8Hiuz


2) The leading hypothesis is that the new variant evolved within just one person, chronically infected with the virus for so long it was able to evolve into a new, more infectious form.

same thing happened in Boston in another immunocompromised person that was sick for 155 days.

3) What happened in Boston with one 45 year old man who was highly infectious for 155 days straight before he died... is exactly what scientists think happened in Kent, England that gave rise to #B117.


4) Doctors were shocked to find virus has evolved many different forms inside of this one immunocompromised man. 20 new mutations in one virus, akin to the #B117. This is possibly how #B1351 in South Africa 🇿🇦 and #P1 in Brazil 🇧🇷 also evolved.


5) “On its own, the appearance of a new variant in genomic databases doesn’t tell us much. “That’s just one genome amongst thousands every week. It wouldn’t necessarily stick out,” says Oliver Pybus, a professor of evolution and infectious disease at Oxford.
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Wonder of Science
Wonder of Science
@wonderofscience
These 'Beach Animals' were created by Theo Jansen as a fusion of art and engineering. The kinetic structures walk on their own and get all their energy from the


Watch the full video on Youtube:
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Adam Hamdy
Adam Hamdy
@adamhamdy
1. I find it remarkable that some medics and scientists aren’t raising their voices to make children as safe as possible. The comment about children being less infectious than adults is unsupported by evidence.


2. @c_drosten has talked about this extensively and @dgurdasani1 and @DrZoeHyde have repeatedly pointed out flaws in the studies which have purported to show this. Now for the other assertion: children are very rarely ill with COVID19.

3. Children seem to suffer less with acute illness, but we have no idea of the long-term impact of infection. We do know #LongCovid affects some children. @LongCovidKids now speaks for 1,500 children struggling with a wide range of long-term symptoms.

4. 1,500 children whose parents found a small campaign group. How many more are out there? We don’t know. ONS data suggests there might be many, but the issue hasn’t been studied sufficiently well or long enough for a definitive answer.

5. Some people have talked about #COVID19 being this generation’s Polio. According to US CDC, Polio resulted in inapparent infection in more than 99% of people. Severe disease occurred in a tiny fraction of those infected. Source:
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Secrets of Ganapati (Author - Keshav Sitaram Thackeray)

This article is taken from Keshav Sitaram Thackeray's book 'Dharmachi Devale and Devalcha Dharma'. In it, Bal Thackeray's father is claiming how the Hindu deity of Ganesha is imaginary.
(1/20)

Buddha is Ashtavinayak and "Ganapati Bappa Morya" is "Chandragupta Morya".

In the era of democracy, the head of state is the President. But in ancient India, there was a Gana culture in the monarchy and the head of that Gana was called Ganapati
(2/20)

In ancient India, a prince named Siddharth Gautam was born in royalty. He later became the king of Shakya Gana in this Gana culture. In time, Siddhartha attained enlightenment.

Let us now understand the difference between a real Ganapati and an imaginary Ganapati.
(3/20)

Some clever Brahmin manuvadis made the real Ganapati the imaginary Ganapati.

Buddha was the king of the Shakya Gana, so people called him Ganaraja. He founded Buddhism and became the head of the Shakyas. As the head of Gana, people used to call Buddha as Ganapati.
(4/20)

But the Brahmins Brahmanized Ganapati and created a false Ganapati. When Buddha used to give the message of Dharma to the people, two words would come out of his message. Chitta and Malla.

Chitta is the body.
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WORLD OF SANATAN DHARMA
WORLD OF SANATAN DHARM...
@world_sanatan
WHY BABUR NEVER TRIED TO ATTACK SRI KRISHNADEVARAYA ?

A🧵u must read

It is very well known that Babur considered Krishna Dev Raya as the strongest ruler of the entire subcontinent, and Vijayanagar empire the strongest during that time in India.

1/10


The Vijayanagar empire reached its peak during 1509-1529 around the reign of Krishna Dev Raya.

Vijayanagar’s famed elephant brigade 👇

2/10


At his command there were over 50000 elite troops with a regiment of Portuguese gunners and 3200 cavalry with 600 elephants.

3/10

Along with that more than four hundred thousand ( four lakh) peasant levies and irregular military made this empire one of the largest in all of South Asia.

A typical Vijayanagar levie soldier 👇

4/10


During Baburs invasion, Krishna Dev Raya ruled supreme in all of deccan.

In fact, in an all our brawl, Krisha Dev Raya will beat Babur fair and square.

Babur could muster at max 50000 troops with 50 canons.

While Krishna Dev Raya had these numbers in Hampi alone

5/10
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Anu Satheesh
Anu Satheesh
@AnuSatheesh5
Dhanwanthari Temple, Nelluvai
Thrissur District, Kerala.🚩🚩

ॐ नमो भगवते वासुदेवाय धन्वन्तरये अमृतकलश हस्ताय
सर्वामय विनाशनाय त्रैलोक्यनाथाय श्री महाविष्णवे नमः
#temples

Dhanwanthary Moorthy temple in Nelluvai is one among the ancient temples in Kerala.


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@SriramKannan77


Smrata-Matrarti-Nasanah', Srimad Bhagavatam states that the one who remembers the name of Dhanwantari will be cured and protected from all the diseases. Temple is known for its Oushada Prasada with Ayuvedic medicinal values, called Mukkudi.


Mukkudi Nivedyam is a very special offering to Dhanwantari. It is prepared by using dry ginger, pepper, pomegranate peel, cumin seed, turmeric, rock salt and other medicinal items. It is offered during 'Usha- Pooja', and is later on distributed to the devotees present there.
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On the occasion of youtube 20k and Twitter 70k members

A small tribute/gift to members

Screeners

technical screeners - intraday and positional both

before proceeding - i have helped you , can i ask you so that it can help someone else too

thank you

positional one

run - find #stock - draw chart - find levels

1- Stocks closing daily 2% up from 5 days
https://t.co/gTZrYY3Nht

2- Weekly breakout
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3- Breakouts in short term
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4- Bullish from last 5

intraday screeners

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6- Intraday Buying seen in the past 15 minutes
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Thank you
SCREENERS , ALL , NAKULSMIX