BC LNP
Saved by @rubic3n

80% of diseases not treatable with 'conventional medicine'. Protein treatment as an alternative with drawbacks.
An 80% market for mRNA. Yet, the vector is the bottleneck causing the limitation.
Viral V is almost a 'use once only' bcs of antibody formation.
LNP sole way to push.

 mRNA Therapy - with a gargantuan market - in dire need for a vector.
Viral vectors as a dead ally until 2015.
mRNA Therapy meets enormous resentments.

Luckily, billions of people have now got first hand experience. Obviously, AZ was not that useful in the general drift.
LNP instead of a vector
PFIZER BioNtech

ALC-0315
ALC-0159, DSPC
"This product is for research use only and not for human use."
https://t.co/tNsG5wxVCT

'EMA Zulassung ohne Daten'
https://t.co/cWW1PDMzW3
Official statement by Echelon
Paraphrased - "we do produce it, but only for use in labs.
Pfizer get theirs from other companies. With them it is safe in humans."

No study data presented
A reference to GMP as 'safe in humans'

ALC-0315
ALC-0159, DSPC
PFIZER LNP

This paper claims "It is just like another fatty compound. You eat salad oil and chips as well"

https://t.co/uncdUpkFnI

No, it is not.
LNP is far from being 'just another salad oil'.

It is
-a recent development
-proprietary
-highly specialized
-very expensive and
-It was one of the limiting factors in delivering the mRNA and not killing people.
https://t.co/VXAK5RNTAs

https://t.co/wC3nGyo7wp
.
The LNP is the hidden 'trouble maker'
It is often left out of explanatory drawings such as this one.
Not because it is not important, but bcs it poses questions:
- how stable is it?
- how is made sure that it only reaches desired target cells - and at the injections site?
As mentioned above, it is a fairly recent field of interest

https://t.co/UH0h4StiXR
A vast area - far from 'just being another salad oil'
- different compositions
- charge
- qualities that allow it to be injected again

Some interesting comments beneath
https://t.co/AImm2MMdOV

Wide use of applications - and specific formulations
https://t.co/1JNg2iWXMJ
.
Are Lipid Nano Particles LNP organ specific?

A discussion for a POTENTIAL FUTURE development. 2021/August Paper
https://t.co/t5yiijYlCr

by
- cationic charge
- embedding target cell specific antibodies
Do LNP stay at the injection site?

The cationic charge is the property that makes it stay longer at the site of injection - however, 'longer' is relative. Animal models?
Any other mechanisms?
https://t.co/TnqGqSSSSs
“Everyone is trying to figure out the next big ionizable lipid"
LNP 'Parts List'
https://t.co/TnqGqSSSSs
https://t.co/1JNg2iWXMJ
LNP seem to have their natural tissue affinity.
Heart muscle cells.
Among others.

https://t.co/phOW7BsMrf

https://t.co/DIWgG5P5SY

https://t.co/lnfOoIKua5
Some producers of vaccines have gone ahead with their own special LNP formulations - which read like a scifi book in part.

Just to name select few:
Acuitas
(Trudeau)

https://t.co/Gv5XFbm6Qj
Moderna has been very inventive in the area of modulating their LNP - it is even designed to enter stem cells, ...

You may want to have a look for yourself.

Moderna LNP - Route of administration

https://t.co/ykXuTk5MhR
.
Moderna LNP modifying cell uptake.

A bit short sighted, when taking into consideration in vivo parameters not present in the reduced model.
Such as:
- Genes can be switched on and of in patterns, diurnally
- with age
- nutrition
- other medication ...

https://t.co/EpGIOcQimJ
.
Moderna LNP
In their high gloss material they were already envisaging serial application

https://t.co/bB2pHGA2CX
.
Moderna LNP

Stem Cell - 'transfecting bone marrow cells'
Going that deep actually would require looking around for other influencing factors more

https://t.co/Wy47iMEWGP
Other studies - already in 2017 - looking specifically at
"repeated applications"
To find the right balance between toxicity and therapeutic value (tv).
Is this what we are observing in the third dose and no tv for children at the moment?

https://t.co/fvlE7lbyIl
Just a reminder - PFAEZER's LNP

https://t.co/YXltgTZaKE
.
LNP
The mRNA-LNP platform’s lipid nanoparticle component used in preclinical vaccine studies is highly inflammatory

https://t.co/gXZ8fCZ5H9
.
For further reading (uncategorized)

https://t.co/GbyRljCNpi
https://t.co/2dMkuO7dyQ
https://t.co/O9MRNhz3ul
https://t.co/4dAMzuGqD8
.
Mix your LNP yourself

https://t.co/crNb4sLyUa
.
Short overview of who is involved in LNP production for PF and Mode RNA

https://t.co/LinrJ8g858
Different LNPs for different applications in clinical trials

Also SPION tech
Magnetic
Carbon

and other weird and wonderful
Link LNP SPION et al
2021 August

Nanoparticles in the clinic: An update post COVID-19 vaccines

https://t.co/tXRJdt8RfO
.

More from rubic3n

rubic3n
rubic3n
@rubic3n
Novavax
Matrix M coating for cancer therapeutics

Following in the orchestrated wave if 'Emerging infectious diseases'.
A good cloak to hide a nasty machinery beneath.

2022
https://t.co/gm76Pzw0KT
further articles in following tweet

2017 Biomerieux
https://t.co/hrCU2xVi3U
2016
https://t.co/ZpyQ4WeUWZ
2016
https://t.co/ua10Y0fu0Z
2015 mice
NOVAVAX
rubic3n
rubic3n
@rubic3n
@Keane1975 Die Forschung mit Virenmanipulation und mRNA Impfstoffen geht dem schon seitlich weit voraus.
Nachfolgend
- zum Bau des Labors in Wuhan durch BioMerieux -> Bancel jetzt Moderna
- Werbebroschüre BioMerieux (CEPI GAVI Einbindung)
- Moderna ominöser geheimer weiterer Impfstoff
1

Bau des Labors in Wuhan BioMerieux Beteiligung
2004


Bancel BioMerieux


Mehr zum Bau des Labors durch BioMerieus in Wuhan (Stephane Bancel ex BioMerieux jetzt


Bancel BioMerieux Wuhan
DARPA BIOMERIEUX MODERNAPDFMAKERAPP GRAB THIS
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rubic3n
rubic3n
@rubic3n
Virus-Like Particles: Revolutionary Platforms for Developing Vaccines Against Emerging Infectious Diseases
https://t.co/gm76Pzw0KT

In memoriam Nicola Bidoli

VLP applications characteristics challenges


VLP Induction of innate and adaptive immunological responses (A) humoral immunity; (B) cell-mediated immunity) by VLPs


VLPs Advantages and limitations of different expression systems for the development of virus-like particles.


VLP vaccines against different viruses and parasites.
VLP
rubic3n
rubic3n
@rubic3n
Fondation Merieux
2017
Pushing the
CEPI Dialogue

Excellent presentation to see, how they, including Bancel, had layed out the narrative.
Quess, what they had specifically included in their list of targeted viruses?

https://t.co/J5FDe6W986


Fondation Merieux 2017
CEPI
Almost basking about their list being more inclusive, more emergent viruses based.
https://t.co/J5FDe6W986


Fondation Merieux 2017
Narrative: "Look, what ZIKA has done to your populace. Nasty ZIKA."

How much of that ZIKA damage was actually related to a virus? Was there a computer guy, who had invested in mosquitos as a vector?

https://t.co/J5FDe6W986


Fondation Merieux 2017
What is the advanges of a programme like CEPI.

And for who?

Let me translate: They want a coordinated vaccine production, with only one strong production pipeline, and solidly regulated pathsways.
Provide the incentives for big pharma.

They've made it.


Vision - look at nasty ZIKA, you surely do not want that
Mission - get the pipeline going of (only) the big ones
Scope - the anti-cancer platforms with their DNA, mRNA basis fit perfectly. They so far do not sell very well, but they could be recycled.

Correct me, if I am wrong.
MERIEUXCEPIPREPARINGTHEMARKET
rubic3n
rubic3n
@rubic3n
@PParzival Es gabe eine parallele Entwicklung mit der Entwicklung der mRNA. Im Packet mit LNP (Lipid Nano Partikeln, den Vehikeln), und Graphene (zur Trennung im Herstellungsprozess, aber auch mögliche andere Effekte wie Tight Junction Öffnung) und der breiten Produktpalette der Hydrogels.

Mit einer nie da gewesenen Hoffnung auf:
- neue industrielle Revolution (meist Graphene basiert, durchsichtig, stretchy, SPIN tech fähig, mit regelbarer Ladung, Magnetizität in ALLEN Lebensbereichen) -damit MEHR PROFIT für die Computerindustrie mit geringeren Entwicklungssprüngen

2011 Ein bedeutendes Jahr mit großem finanziellen Commitment der großen
2012 George Church Emergency Summit on rogue self-replicating Nano Bots (da noch nicht eingetreten, aber den Giftkochern schon bewußt)
2015 Buy-in in alle großen mRNA Impfstoff Platformen.

Auch 2015, PR roll-out Gates Concept Designerin Health (Impfstoffe und Weltkörperkontroll Organisation (natürlich nennen sie es anders, übrigens nicht WHO) Sue Desmond-Hellmann
2016 großer Moderna Niederschlag mit tödlichen Vehikel
Gleichzeitig Push neue LNP Entwicklung

2018 Baric plug in your spike Lego Technik einsatzbereit. Gates Munich Security Conference 'Es wird einen Terroristischen Virenanschlag geben'; Fauci 'There is going to be a surprise outbreak'
2018 Enigma Lieferung nach Wuhan, damit zweite 'No see um' DNA RNA Technologie für Lego
IR4

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David Rothkopf
David Rothkopf
@djrothkopf
If there is one thing I have learned it is that people love bullshit. They buy bullshit stories from professional bullshit artists, accept bullshit philosophies, purchase bullshit how-to-books, vote for bullshitting politicians.

At first, I resisted believing this because it is illogical and dumb and frankly, doesn't reflect well on the human race of which I am a member (and thus have long been hopeful that it is more of a distinction than it actually turns out to be.)

But if you actually are interested in facts you begin to notice how little most people care for them. Sometimes it is because they are heard to learn and bullshit is so much more widely and easily available. Sometimes it is because facts make them uncomfortable about themselves.

Sometimes it is because just one tiny fact will upset a whole worldview that has grown as comfortable as an overstuffed chair. (Settling into such worldviews is a lot like settling into chairs like that because the longer you're there and the deeper you sink...

...the harder it is to hoist yourself up and out of your fat-assed intellectual laziness.) But I actually think the reason people have such an appetite for bullshit goes deeper, goes to an aspect of ourselves we just can't accept.
LIFE
Vibhu Vashisth
Vibhu Vashisth
@VIBHU_Tweet
Did you know?

When Saudi Arabia's King visited India in 1955, PM Nehru ordered all the Hindu Temples and Idols of Hindu Deities that came in Saudi King's way to be covered up.
Our temples are the architectural marvels in their own league.


Each of our temples tells the story of our rich history and cultural heritage. Was Nehru that ashamed of his own nation's heritage that he had to cover them up or there was some other reason? The answer would be best known to him only.

PM Nehru invited the then Saudi King Saud bin Abdul Aziz to India who accepted the invitation and planned a 17 day long official visit to India in 1955. Saudi King had brought a large delegation with him and he had to visit various Indian cities like Mumbai, Hyderabad, Agra,


Aligarh, Shimla, Mysore and Varanasi. However, when Saudi King reached Varanasi, Nehru ordered to cover all the temples and Idols with curtains that came in the way of the Royal Convoy. During his stay in Varanasi, Govt buildings were painted with the words "Kalma Tayyiba".


'Kalma Tayyiba' means 'There is not God but only Allah and Mohammed is his messenger'. In addition to it, Pooja and Aartis were also stopped at temples. I can call it the appeasement politics of the highest order and Nehru was indeed the greatest appeaser of all times.
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Pran Katariya
Pran Katariya
@KatariyaPran
The Straddle is my favorite trade these days. But more often than not, one needs to adjust to make a good return from it. There are many ways to do this depending on your view, psychology and imagination. Will discuss a few that I use

The Straddle is a delta neutral strategy and it is important to keep deltas in check during the course of the Trade and not let them run away from you. Of course Volatility is the elephant in the room- but not going there as that will require a separate discussion

The first and most common method is to go 'inverted.' That is to say to shift the untested side to generate additional Premium and increase the runway to Breakeven. So if you start with a Straddle at 700 and price goes to 740, you can move the PE up to say 720.

How much should you move the untested side? While again this is a matter of skill, experience and artistry, the rule of thumb is to cut deltas by half. Remember, prices will move and you have to allow sufficient room for them to fluctuate

The second method is to shift the entire Straddle in the direction of the move. So if you have a 700 Straddle and Price goes to 740, you can shift the Straddle to 720. If you time this right, it can generally be done at virtually no cost or minimal cost.
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Dr. Thomas Binder, MD
Dr. Thomas Binder, MD
@Thomas_Binder
1/12

RT-PCR corona (test) scam

Symptomatic people are tested for one and only one respiratory virus. This means that other acute respiratory infections are reclassified as


2/12

It is tested exquisitely with a hypersensitive non-specific RT-PCR test / Ct >35 (>30 is nonsense, >35 is madness), without considering Ct and clinical context. This means that more acute respiratory infections are reclassified as


3/12

The Drosten RT-PCR test is fabricated in a way that each country and laboratory perform it differently at too high Ct and that the high rate of false positives increases massively due to cross-reaction with other (corona) viruses in the "flu


4/12

Even asymptomatic, previously called healthy, people are tested (en masse) in this way, although there is no epidemiologically relevant asymptomatic transmission. This means that even healthy people are declared as COVID


5/12

Deaths within 28 days after a positive RT-PCR test from whatever cause are designated as deaths WITH COVID. This means that other causes of death are reclassified as
CATEGORY C19
Vibhu Vashisth
Vibhu Vashisth
@VIBHU_Tweet
प्राचीन काल में गाधि नामक एक राजा थे।उनकी सत्यवती नाम की एक पुत्री थी।राजा गाधि ने अपनी पुत्री का विवाह महर्षि भृगु के पुत्र से करवा दिया।महर्षि भृगु इस विवाह से बहुत प्रसन्न हुए और उन्होने अपनी पुत्रवधु को आशीर्वाद देकर उसे कोई भी वर मांगने को कहा।


सत्यवती ने महर्षि भृगु से अपने तथा अपनी माता के लिए पुत्र का वरदान मांगा।ये जानकर महर्षि भृगु ने यज्ञ किया और तत्पश्चात सत्यवती और उसकी माता को अलग-अलग प्रकार के दो चरू (यज्ञ के लिए पकाया हुआ अन्न) दिए और कहा कि ऋतु स्नान के बाद तुम्हारी माता पुत्र की इच्छा लेकर पीपल का आलिंगन...

...करें और तुम भी पुत्र की इच्छा लेकर गूलर वृक्ष का आलिंगन करना। आलिंगन करने के बाद चरू का सेवन करना, इससे तुम दोनो को पुत्र प्राप्ति होगी।परंतु मां बेटी के चरू आपस में बदल जाते हैं और ये महर्षि भृगु अपनी दिव्य दृष्टि से देख लेते हैं।

भृगु ऋषि सत्यवती से कहते हैं,"पुत्री तुम्हारा और तुम्हारी माता ने एक दुसरे के चरू खा लिए हैं।इस कारण तुम्हारा पुत्र ब्राह्मण होते हुए भी क्षत्रिय सा आचरण करेगा और तुम्हारी माता का पुत्र क्षत्रिय होकर भी ब्राह्मण सा आचरण करेगा।"
इस पर सत्यवती ने भृगु ऋषि से बड़ी विनती की।


सत्यवती ने कहा,"मुझे आशीर्वाद दें कि मेरा पुत्र ब्राह्मण सा ही आचरण करे।"तब महर्षि ने उसे ये आशीर्वाद दे दिया कि उसका पुत्र ब्राह्मण सा ही आचरण करेगा किन्तु उसका पौत्र क्षत्रियों सा व्यवहार करेगा। सत्यवती का एक पुत्र हुआ जिसका नाम जम्दाग्नि था जो सप्त ऋषियों में से एक हैं।
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