BC HEALTH
Saved by @Alex1Powell

https://t.co/n7fagVLYm0
We assembled two mammalian expression vectors and one DNA cassette inserted into African Swine Fever from PRJNA607174! What happened in GuangDong at Mar-Aug 2019???!

 The legitimacy of those “samples”—completely destroyed. The CoV-like sequences—cloned. No data from the pCoV group should ever be trusted in any way anymore!
Note: the DNA cassette exist in both unintegrated and integrated forms. Likely using homology-directed recombination. Whatever they were trying to express it is not just one or two proteins. There were also SV40 Ori which is yet to be properly mapped.
https://t.co/O1FYnwX6Oj
Why you need expression vectors in VERO since these cells are never used as expression hosts? Especially since there were a load of different tags on these vectors. The proteins had novel tags both N and C, IgK, His, Myc—especially His tag. This is for
NiNTA purification. There is no way that anyone would tag a protein this way and only use it to transfect VERO cells. There are no other host cells in these datasets other than Manis Javanica. Only Manis Javanica and Chlorocebus Aethiops. VERO is never used for recombinant
Protein production, and there were no pigs in the dataset. Vectors therefore intended for Manis Javanica cells. This mean that the GD pangolins were intentionalally transfected with expression vectors and inoculated with recombinant ASFV expressing most likely CoV antigens.
GFP can’t even pass through the secretion pathway—why fuse that behind an IGk secretion signal?
SV40 pA.
Did they GFP-tag some of these proteins and make a recombinant ASFV just to make up the symptoms and histopathology? Very likely!
ASFV particles are found in Double-membraned vesicles when assembled, ASFV particles with GFP tagging glows green granularly in tissue, ASFV causes pneumonia and thromboembolism—it is the perfect body double for a fake CoV in all and every “supporting picture” they made!
Inoculate with rASFV. Mix in some CoV pEASY-T1 clones. Add in pcDNA3.1+/SV40pA-S-E-M-N. You get the perfect fake sample for a fake CoV!
@DrLiMengYAN1
Why there were no 5’- and 3’- sequence for MP789? The SRA had these junctions erased.
So to not be immediately obvious. But the other vector backbone sequences were left behind.
Synthetic sequences invalidate the supposed origin of the samples. Either Reused VERO stock or clone-derived sequences created the trace CoV reads.

More from Health

First Doctor
First Doctor
@firstdoctorr
ECTOPIC PREGNANCY

A woman just lost her life due to ectopic pregnancy!

It's a pregnancy in which the embryo attaches outside the womb. It can be life-threatening.

But we can save lives by helping educate women on our Timelines with this.

THREAD 🧵


In the human body, a fertilized egg hardly survives outside the womb. If allowed to grow, it may damage nearby organs & cause severe blood loss.

The bulk of ectopic pregnancies occur in/along the Fallopian tubes.

Some in the ovaries, cervix or a random location in the abdomen.


The usual symptoms of ectopic pregnancy a woman may experience esp. in early pregnancy are;

* Abdominal pain
* Vaginal bleeding, &
* Amenorrhea (missing your period)

Other pregnancy symptoms like breast fullness & occasional nausea may be there too.

But the common DANGER SIGNS of ectopic pregnancy that may signify rupture are;

🔴 Rigid abdomen

🔴 Severe abdominal pain

🔴 Shoulder tip pain, &

🔴 Signs of shock such as cold hands & feet.

With these signs, immediate medical attention is needed to save the woman's life!

Your chances of having an ectopic pregnancy are higher if you have;

1. Previous ectopic pregnancy

2. STI's like gonorrhea

3. Fertility treatments like IVF*

4. Fallopian tube surgeries

5. Pregnancy while using an Intrauterine Contraceptive Device

6. Smoking history! 🚭
HEALTH
Gregg Gonsalves
Gregg Gonsalves
@gregggonsalves
Eight years ago, Spencer Cox died of #AIDS. He had access to #antiretroviral therapy, but in his own struggles with #depression and substance use, he stopped taking them. 1/

Spencer left college, dropped out like me in the 80s, and we both found ourselves in @actupny where we joined the Treatment and Data Committee, doing battle with drug companies, the @US_FDA, the @NIH on a quest to save ourselves and our friends from near-certain death. 2/

Now everyone knows what those dark days before the advent of antiretroviral therapy were like. Waves upon waves of dying friends. A government who couldn't care less for the sick and dying. 3/

All this has happened before, and all this will happen again. AIDS. #COVID19. The next pandemic. The fire next time. 4/

And it changes you. Seeing so much death so young. Seeing those in power turn away from the catastrophe, even joke about it (find, listen to the tape of Larry Speakes in Oval Office w Reagan laughing about AIDS). 5/
HEALTH
Advertisement
Nick Hinton
Nick Hinton
@NickHintonn
A Conspiracy Thread: The Sentient World Simulation


In 2007, the Department of Defense worked with researchers at Purdue University to create SEAS, or the Synthetic Environment for Analysis and Simulations. SEAS uses data from the internet to create realistically detailed models of the world which are used to predict the future.


We’re told SEAS is now capable of running real-time simulations of up to 62 nations and that it’s been used by Fortune 500 companies for strategic planning, or in other words, manipulating the market. Nevertheless, there are plans to turn SEAS into something even more advanced.


Developers say their goal is to turn SEAS into a Sentient World Simulation, or a “continuously running, continually updated mirror model of the real world.” However, some conspiracy theorists claim the SWS is already live and running inside a network of quantum computers.


Researchers like Seth Lloyd have suggested that the entire universe could be simulated inside a quantum computer. According to Geordie Rose, the co-founder of D-Wave Systems, a company that creates these machines, they can even tap into parallel worlds and other dimensions.
HEALTH , ALL
Yohannes #Tigray First
Yohannes #Tigray First
@Yohannes_T_M
@MSF was allowed to enter #Mekelle on 16 Dec & here is their account 👇

"no basic supplies. The local hospital was running at 30 to 40 %, with very little medication"

This is why one of the reason why we demand unfetters #HumanitarianCorridor
#TigrayGenocide

#Adigrat "The situation was very tense & its hospital was in a terrible condition. Most of the health staff had left, there were hardly any medicines &there was no food, no water and no money"
#StopWarOnTigray
#TigrayGenocide
#HumanitarianCorridor
#EritreaOutOfTigray

After 1 month since @AbiyAhmedAli declared victory

"#Adwa & #Axum. There we found a similar situation: no electricity & no water. All the medicines had been stolen from #Adwa general hospital & the hospital furniture & equipment were broken"

#StopWarOnTigray
#TigrayGenocide


"Beyond the hospitals, around 80 or 90 per cent of the health centres that we visited between Mekele and Axum were not functional, either due to a lack of staff or because they had suffered robberies"

#StopWarOnTigray
#TigrayGenocide
#EritreaOutOfTigray
@UN @POTUS @StateDept


"1 woman had been in labour for 7 days without being able to give birth. Her life was saved because we were able to transport her to #Mekele"

It is really heartbreaking to imagine how many of our mothers & sister have lost their lives.

#StopWarOnTigray
#TigrayGenocide
@UN
HEALTH
\u2744\ufe0fI'm A Fuckin' Princess\u2744\ufe0f
❄️I'm A Fuckin' Prince...
@Just_ReneaR
I'm not pro-murdering babies.
I'm pro-Becky who found out at her 20 week anatomy scan that the infant she had been so excited to bring into this world had developed without life sustaining organs.
HEALTH

You May Also Like

Pieter Levels \u2728
Pieter Levels ✨
@levelsio
Joe Rogan's podcast is now is listened to 1.5+ billion times per year at around $50-100M/year revenue.

Independent and 100% owned by Joe, no networks, no middle men and a 100M+ people audience.

👏

https://t.co/RywAiBxA3s

Joe is the #1 / #2 podcast (depends per week) of all podcasts

120 million plays per month source https://t.co/k7L1LfDdcM


https://t.co/aGcYnVDpMu
MAKERS
Seth Abramson
Seth Abramson
@SethAbramson
(MAJOR NOTE) The reason Manafort took over Trump's campaign in just 3 weeks despite being hired as a mere delegate counter is that ROGER STONE initiated a whisper campaign against Corey Lewandowski. That campaign started...days after Manafort returned from meeting with WikiLeaks.

2/ Stone and Manafort are longtime business partners; we'd expect Manafort to tell Stone what Assange told him, and for him to expect Stone to then pressure the campaign not only to hire him but quickly elevate him.

And who was Stone in touch with at the campaign? Trump himself.

3/ I bet you Manafort's late March emails are very interesting and that some of them are to/from his longtime business associate Roger Stone. And I bet Roger Stone's late March phone calls are very interesting and that some of them are to/from his longtime friend Donald Trump.

4/ At the end of March 2016, Trump suddenly convenes a NatSec meeting. At that meeting he directs his NatSec team to change the RNC platform in July to benefit Putin. Who later takes credit for that change?

Paul Manafort's business associate, Kremlin agent Konstantin Kilimnick.

5/ Why did Donald Trump suddenly convene a NatSec meeting 3 days after he finally hired Manafort? Why did he issue a pro-Kremlin directive at that meeting that Manafort's camp would later take credit for? What did Manafort tell Trump in the days leading up to that NatSec meeting?
POLITICS
Advertisement
Loren Brichter
Loren Brichter
@lorenb
So it turns out that Google Chrome was making everything on my computer slow *even when it wasn’t running*, because it installs something called Keystone which is basically malware.

I made a website because this shouldn’t

Wired first reported on how bad Keystone was 11 years ago when they put it into Google Earth (they seem to put it in all their popular

The fact that Keystone hides itself in Activity Monitor is bizarre. (The only sign of it was excessive CPU usage of WindowServer which is a system process).

I don’t know if Google was doing something nefarious with Keystone, or a third party figured out how to (which Wired warned about). But either way, I’m not inclined to give Google-the-organization the benefit of the doubt (despite the many good people who work on Chrome)...

...since it's been a decade+ and this still hasn't been "fixed".

There is no reason for auto-update software to need to do what Chrome/Keystone was doing. It also has a long history of crashing Macs.
TECH
V\u0936\u0941\u0926\u094d\u0927\u093f
Vशुद्धि...
@V_Shuddhi
क्या आप जानते हैं कि क्या है, पितृ पक्ष में कौवे को खाना देने के पीछे का वैज्ञानिक कारण!

श्राद्ध पक्ष में कौओं का बड़ा ही महत्व है। कहते है कौआ यम का प्रतीक है, यदि आपके हाथों दिया गया भोजन ग्रहण कर ले, तो ऐसा माना जाता है कि पितरों की कृपा आपके ऊपर है और वे आपसे ख़ुश है।


कुछ लोग कहते हैं की व्यक्ति मरकर सबसे पहले कौवे के रूप में जन्म लेता है और उसे खाना खिलाने से वह भोजन पितरों को मिलता है

शायद हम सबने अपने घर के किसी बड़े बुज़ुर्ग, किसी पंडित या ज्योतिषाचार्य से ये सुना होगा। वे अनगिनत किस्से सुनाएंगे, कहेंगे बड़े बुज़ुर्ग कह गए इसीलिए ऐसा करना

शायद ही हमें कोई इसके पीछे का वैज्ञानिक कारण बता सके।

हमारे ऋषि मुनि और पौराणिक काल में रहने वाले लोग मुर्ख नहीं थे! कभी सोचियेगा कौवों को पितृ पक्ष में खिलाई खीर हमारे पूर्वजों तक कैसे पहुंचेगी?

हमारे ऋषि मुनि विद्वान थे, वे जो बात करते या कहते थे उसके पीछे कोई न कोई वैज्ञानिक कारण छुपा होता था।

एक बहुत रोचक तथ्य है पितृ पक्ष, भादो( भाद्रपद) प्रकृति और काक के बीच।

एक बात जो कह सकते कि हम सब ने स्वतः उग आये पीपल या बरगद का पेड़/ पौधा किसी न किसी दीवार, पुरानी

इमारत, पर्वत या अट्टालिकाओं पर ज़रूर देखा होगा। देखा है न?

ज़रा सोचिये पीपल या बरगद की बीज कैसे पहुंचे होंगे वहाँ तक? इनके बीज इतने हल्के भी नहीं होते के हवा उन्हें उड़ाके ले जा सके।
ALL
Simon Wardley #EEA
Simon Wardley #EEA
@swardley
X : Our executive team is concerned that we need to up our game in order to out innovate Amazon.
Me : Do you map?
X : Yes
Me : Like this?
X : No. What's that?
Me : A map of a tea shop.


X : Why is that a map?
Me : Long story, all to do with how space has meaning. To keep it short, maps help people to focus on user needs, the components involved, to communicate missing components and scenario play ideas like staff becoming robots.


Me : They’re also good for measuring and managing capital flow, making investment decisions, removing bias and getting rid of duplication.
X : I don’t see how that helps with innovation?


Me : Well, innovation is a tricky word because we use it to describe many things. If you’re talking about differentiation with the adjacent unexplored then you’re experimenting in the “uncharted” space e.g. immortality with magic provided by the special custom built kettle.


X : That sounds like nonsense.
Me : A lot of what people think will be the next great innovation is nonsense. Actually, most of it is. That’s the nature of the uncharted space, it’s experimental, high risk and generally results in failure.
X : We need more reliable innovation.
TECH