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Saved by @BillyBostickson

Compilation Thread on ORF8 ORF8 or Fate?
1. ZLS (2018):
"Considering the variability of orf8 in bats, civets and humans, investigating the function of orf8 is a priority, particularly the contribution of these different variants to viral pathogenesis."

 2. ORF8 or Fate?
"In addition to receptor binding, proteolytic cleavage of S & potentially other mutations that affect virion & trimer stability may be important for virus transmissibility in different hosts, & these factors need to be studied further."
https://t.co/oNHpIsiM4p
3. ORF8 or Fate?
Overwhelmingly these viruses had mutations or
29 deletions in ORF8 - associated with reduced replicative fitness of the virus.. may be associated with host adaptation
via @CarltheChippy
382-nt deletion during early evolution of SARS-CoV
https://t.co/W5IQwDunCN
4. ORF8 or Fate?
@franciscodeasis reviewed the list of read filenames according to dates and labels:
- Sat 2017-06-03 (7896)
- Sat 2017-06-17 to Tue 2017-06-20 (7896 and ORF8)
- Thu 2018-09-27 to Sun 2018-09-30 (RaTG13)
- Mon 2018-10-08 to Sun 2018-10-14 (no label and TSS)
5. ORF8 or Fate?
What is ORF8? This is Orf8!
https://t.co/21ugtiinhL
6. ORF8 or Fate? keep on saying it!
orf8 NSP12 deletion found in India
https://t.co/1TQ9ftuToV
https://t.co/ysNXp88dWf
7. ORF8 or Fate?
Via @ConsciousEvolu1
What is critically important is that Orf8 and surface Glycoproteins create Porphyrin penetration of the Cell. Resulting in more Severe Infections, Bio-chemic Processes interuption, Heme cleavage and Hypoxia.
https://t.co/XyiU1879Rq
8. ORF8 or Fate?
via @Rossana38510044
"The ORF8 Protein of SARS-CoV-2 Mediates Immune Evasion through Potently Downregulating MHC-I"
https://t.co/m9KY0Fkxkx
9. ORF8 or Fate?
"Immune evasion via SARS-CoV-2 ORF8 protein?"
https://t.co/oEncCgDDvb
10. ORF8 or Fate? Stress Trigger!
"However, 2 proteins (orf8 & orf10) in SARS-CoV-2 have no homologous proteins in SARS-CoV. The amino acid sequence of orf8 in SARS-CoV-2 is different from sequences of conserved orf8 or orf8b derived from human SARS-CoV"
https://t.co/Y76d3b3sH3
11.ORF8 or Fate! Coincidence? No, Trust a Me, it's Natural!
1. Orf8 "combined" with Orf10 to create a Porphyrin Heme Cleave and Orf8 Porphyrin Infectivity
2. WIV were already studying the RaTG13 and 7896 Clade Spike Proteins and Orf8 back in 2018

,
12. via @CZilcho
not a single live virus. genome looks like the version of a "virus" that has been compiled from known RaTG13 contigs & consensus seq from RmYN02 (ORF 1ab, 3a, E, M, 6, 7a) + some unknown seq (passaged viruses?) for S, & 3' from ORF8, N, using Shiver or similar.
13. ORF8 or Fate? ORF8 similarities
Discovery of a rich gene pool of bat SARS-related coronaviruses provides new insights into the origin of SARS coronavirus
Ben Hu (2017)
https://t.co/foBWRXc0Fb
Peppered with 91 references to Orf8
14. ORF8 or Fate? WIV ORF8 Experiments (2017)
Same paper as above
https://t.co/MMO6Br7XQK
15. ORF8 or Fate?
1. ORF8 enhances the viral replication.
2. enhanced ORF8s are main factors contributing to transmission, virulence and host adaptability of CoVs.
3. enhanced ORF8s increase the efficiencies in viral entry into cells and replication

https://t.co/spkLguszNZ
16. ORF8 or Fate?
Back in Time to an ORF8 rich bat cave with WIV and @amicocolorido
Distribution of SARSr-CoVs highly similar to SARS-CoV in the variable S, ORF3 and ORF8 genes in the single cave.
https://t.co/1jU1PaWVoP
17. Some naughty but nice ORF8y images collated by @BidoliNicola
https://t.co/8RQNrSndAs
18. ORF8 or Fate? Back to SARS
https://t.co/PDxyBfpGVg
Severe Acute Respiratory Syndrome (SARS) Coronavirus ORF8 Protein Is Acquired from SARS-Related Coronavirus from Greater Horseshoe Bats through Recombination
https://t.co/c5Sxc23SiG
19. ORF8 or Fate?
Latest Findings - ORF8's secrets exposed
" two unique regions: one that is only present in SARS-CoV-2 and its immediate bat ancestor, and one that is absent from any other coronavirus,"
https://t.co/W1xdkYNBRc
20. ORF8 or Fate? WTF is this?
Paper referenced in above article:
The odd structure of ORF8: Mapping the coronavirus protein linked to disease severity
https://t.co/KXtjwScOJ3
21. ORF8 or Fate? via @Harvard2H
Divergence between Orf7a & Orf8 is exceptionally idiosyncratic, as Orf7a is more constrained, whereas Orf8 is subject to rampant change, a peculiar feature that may be related to hitherto unknown viral infection strategies
https://t.co/rskaNzlbXC
22. "Barring a Technical Artefact"
"Protein interactions of SARS-CoV-2 exhibit such a sharp contrast between Orf7a and Orf8 that, barring a technical artifact with respect to coverage..Orf7a generate variants, such as Orf8, wreaking havoc through immune evasion in the host cell"
23. ORF8 or Fate? via @Undergroundsar3
ORF8 is involved somehow in apoptosis/viral cytolysis pathway and functions to optimize spread of virons while cloaking the infected host cell from CD8+ killer T-cell surveillance.
24. ORF8 or Fate? @SBgrid
1. Solved structure of SARS-CoV-2 ORF8 uncovers vital role in immune suppression & evasion
https://t.co/DNHkoOG9it
2. Covalent dimer is an "evolutionarily" recent addition unique to SARS-CoV-2
3. SARS-CoV-2 ORF8 contains a large, unstructured insertion
25. ORF8 or Fate?
Question for Hans Kristian from @Undergroundsar3
What are your thoughts on the CD8+ t-cell story in the milieu of the host (vs. test tube humoral only neutralization assays) where ORF8 is expressed?
https://t.co/pka3kgY9kA
Referenicing:
https://t.co/cc4hZdk3SY
26. Added Bonus: DIY ORF8
https://t.co/R5ZUPJNMFf
27. ORF8 or Fate?
Evolutionary dynamics of the SARS-CoV-2 ORF8 accessory gene
https://t.co/R2PvNsVleu
28. ORF8 or Fate?
Characterization of accessory genes in coronavirus genomes
https://t.co/Rwehz9SEDS
29. ORF8 or Fate? To be or not to be?
SARS-CoV-2 variants combining spike mutations & absence of ORF8 may be more transmissible & require close monitoring
https://t.co/yWNSdWqMBr
SARS-CoV-2 phylogeny highlighting ORF8-deficient variants resulting from nonsense mutation E64stop.

More from Billy Bostickson 🏴👁&👁 🆓

Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193
Billy Bostickson 🏴👁&👁 ...
@BillyBostickson
November 3 - 9, 2019 -Wuhan Institute of Virology
International Training Course on Biosafety Laboratory Management and Technology
1. Daniel Feakes, head of UN Biological Weapons Convention implementation support agency
2. René Courcol, quality control system expert, France's BSL


List of Lecturers with short bios at
November 3 - 9, WIV Training Course: https://t.co/Bsd9yO40JP
YUAN Zhiming Professor, Wuhan Institute of Virology, CAS (Deputy Director)
SONG Donglin, Professor, Wuhan Institute of Virology, CAS ( Deputy Director of Wuhan BSL-4 Laboratory.)

Dr. TONG Xiao Senior Engineer at WIV (Molecular Virology & (HIV) infection, mechanical & vaccine immunology) On-site Manager & Chief engineer for WIV BSL-4 Lab construction, maintenance & biosafety equipment R&D.

Zhengli Shi, Director of WIV Center for Emerging Infectious Diseases, now in charge of scientific activity in BSL3 & BSL4. Viral pathogen discovery through sequencing techniques, wildlife-borne viral pathogens, particularly bat-borne viruses since 2004.

DENG Fei, Principle Investigator in Research Group on Viruses Resources & Biotechnology in WIV, also Director of Microorganisms & Viruses Resource Center.
Graduate of ECUST, went to Great Lakes Forestry Center, Canada in 2001 & Wageningen University, NL to study baculovirus.
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Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193
Billy Bostickson 🏴👁&👁 ...
@BillyBostickson
Enough Jaw Jaw, this is War!
1. People's Justice Courts to be set up worldwide
2. Citizens' arrests of all Identified Suspects
3. Lab Inspections
3. Harsh interrogation using MKULTRA
4. Public Show Trials video streamed live
5. Cross Questioning of suspects via Twitter & Zoom
6.


A growing list of suspects to be interrogated


Foul Traitors, Charlatans, Frauds, Bat Molesters! Frankensteins, Well Poisoners, Plotters, Deceivers!
Dr. Shipmans, Saboteurs, Devils in White Coats!


Organisations to be Investigated
WIV
Wuhan University IMA
WIBP
WCDC
BEIJING IPB
Nanjing Military Lab
SinoPharm
BARDA
NIH
EMERGENT BIOSOLUTIONS
BIOPORT
GILEAD
SIAC
QTEL
USAMRIID
FORT DETRICK
DARPA
DTRA
Metbiota
ONE HEALTH
GVP
ECOHEALTH
MITRE
BATTELLE
TUNNELL
LAURIMA GOV SERVICES


Suspects (1)
China
Zhengli Shi
Peng Zhou
George Gao
Deyin Guo
Major General Chen Wei
United States
ALEX AZAR
JONNA MAZET
ALISON O’TOOLE
ANTHONY FAUCI
BOYD YOUNT
DAVID FRANZ
DAVID KARESH
DENNIS CARROLL
DYLAN GEORGE
GEORGE PAINTER
IAN LIPKIN
JENS KUHN
JEROME HAUER
JONATHAN EPSTEIN
WORLD , ALL
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Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193
Billy Bostickson 🏴👁&👁 ...
@BillyBostickson
@snip96581187 @Daoyu15 @lab_leak @walkaboutrick @ydeigin @Ayjchan @franciscodeasis @TheSeeker268 @angie_rasmussen Clearly, because as I have been saying for 8 months now, DTRA and DARPA have been using Ecohealth and UC Davis to collect novel pathogens for gain of function work back in the USA. I have documented this in many threads which I will post here just to annoy everyone.

@Daoyu15 @lab_leak @walkaboutrick @ydeigin @Ayjchan @franciscodeasis @TheSeeker268 @angie_rasmussen


@Daoyu15 @lab_leak @walkaboutrick @ydeigin @Ayjchan @franciscodeasis @TheSeeker268 @angie_rasmussen


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@Daoyu15 @lab_leak @walkaboutrick @ydeigin @Ayjchan @franciscodeasis @TheSeeker268 @angie_rasmussen
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Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193
Billy Bostickson 🏴👁&👁 ...
@BillyBostickson
1/ 10
My Tedious Research Thread on Wuhan Seafood Market Positive Environmental Samples and possible links to Animals
Search query: "market" "animal" on cloned github database (deleted on github due to CCP) here:

29/30
Hubei Daily (2015) reported that Zhou Zhanhong, former party group member & chief economist of Wuhan Municipal Administration of Industry & Commerce was arrested on suspicion of abuse of power & bribery, but no trial information has been seen
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Billy Bostickson \U0001f3f4\U0001f441&\U0001f441 \U0001f193
Billy Bostickson 🏴👁&👁 ...
@BillyBostickson
1. Finally!
I found the original H1N1-H5N1 "novel" viruses emails! Yes!
https://t.co/MoPgxYJIEv
Kawaoka Busted!

2. Kawaoka Busted!
More email evidence here:
More Smoking Gun Emails From Kobe University Virologist Teridah Ernala Ginting
Acts Of Espionage Against Indonesia by Akiko Makino and Others From Kobe University's "Secret"

3. NAMRU 2 Indonesia
(where Kawaoka organised theft of viruses)
(Now moved to Phnom Penh)
https://t.co/snRmicOIGN
History:
https://t.co/vEjltSDTOj
Perspectives on the DOD Global Emerging Infections Surveillance System:
GEIS at NAMRU 2, Indonesia (PDF)
https://t.co/SKm22oqBD4


4. NAMRU 2 Indonesia Controversy (2008)
Indonesia Suspends US NAMRU Lab Operations
https://t.co/kLW3zhiDVz
Supari On NAMRU-2
https://t.co/0wPbF4TpTJ
Indonesian Lawmakers Call For NAMRU-2 Probe
https://t.co/S4zZ4TaksW
NAMRU-2 Debate Turns

5. NAMRU Indonesia Controversy Part 2
U.S. insists on immunity for all Namru-2 laboratory staff
https://t.co/KelkEqJQTn
A "fact sheet" on NAMRU-2
https://t.co/Z08aLEDIJ4
US Embassy Half Truths:
https://t.co/b0nP0aVIwV
Indonesia: More attacks on
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Nikita Poojary
Nikita Poojary
@niki_poojary
Catch me if you can @Mitesh_Engr

Time for a🧵
Mitesh Sir's EXPIRY Option Selling 101:
• What to look for?
• Strike Selection & Ratios
• SL mgmt
• Avoiding freezes
• Monthy Expiry
• Event days
• How he would have traded last expiry?

In collaboration with @AdityaTodmal


Edward de Bono says intelligence is like a🚗 Automobile

“A powerful car may be driven badly. A less powerful car may be driven well. The skill of the car driver determines how the power of the car is used.”


What @Mitesh_Engr Sir looks at for Expiry Trading:

• Technicals of BNF
• Individual Heavyweight stocks of BNF
• Support/Resistance of BNF on weekly TF
• Also has Superb SL management
• When it comes to adjustments, he is always on toes


How to Select Strikes to Sell?

• You can't sell based on premium
• Follow only trend
• Price action is best to sell on expiry day
• Never wait for the options to go to zero


Using Ratios to Sell:

• If BNF is in a range then strangles
• If breakout/ breakdown from a range then directional
• Selling 70/30 or 80/20 ratios
• 2 examples on how he used ratios
ALL , MITESH PATEL OPTIONS STRAT , MITESH PATEL EXPIRY GUIDE
Vibhu Vashisth
Vibhu Vashisth
@VIBHU_Tweet
Did you know?
Ahilyabai Holkar was one of India's
greatest women rulers of all time.

Ahilyabai was Born on May 31,1725 in the village of Chondi,Jamkhed distt,Maharashtra to a man named Mankoji Shinde,who was the Patil(Chief) of a village and a member of proud Dhangar Community.


Despite the fact that girls' education was a farther dream in those days, Ahilyabai was homeschooled by her father.
Ahilya didn't belong to any royal family but in a twist of fate she is still remembered as one of the most revered Queen in the history.


While they were living their humdrum lives, one day Malhar Rao Holkar, commander of the Peshwa Bajirao & Lord of the Malwa stopped in Chondi on his way to Pune, where he couldn't help but notice a young 8yrs old Ahilyabai in the temple service feeding the poor & hungry.

He was so impressed by the sheer innocence&beauty with simplicity character of Ahilyabai dat he asked her hand in marriage for his son Khanderao Holkar.Just like that she got married to Khanderao in 1733 at a tender age of 8&became a bride of esteemed Maratha community of Holkars

But destiny had something else in store for her as her husband died in the Battle of Kumbher in 1754 leaving her widow at 29.Distressed Ahilyabai was to commit Sati when her father-in-law Malhar Rao refused to let it happen.
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Vibhu Vashisth
Vibhu Vashisth
@VIBHU_Tweet
RISHI PINGALA:THE ANCIENT INDIAN SAGE DISCREDITED FOR HIS MAJOR CONTRIBUTIONS TO MATHEMATICS & PHI

Have you ever heard about,"The Golden Ratio"?
The Golden Ratio or Phi(Greek) is a mathematical ratio of nature. Mother Nature is the most exquisite artist in herself.


&Its this Phi she uses to create all the beauty in World.
If anyone uses this ratio correctly in their art,it can turn into a spell-binding piece of work.Such is the significance of'Phi'.But my thread isn't about it.Its about somethin called'Fibonacci Series'&who actually made it


Phi is related to Fibonacci series(0,1,1,2,3,5,8,13,21,34...)
&this series is responsible for everything dat is beautiful on this planet. Just try to calculate the ratio of any 2 successive numbers ÷ each of them by the number before it in above infinite series.

E.g. 3/2=1.5, 5/3=1.666, 8/5=1.6, 34/21=1.61904 and so on. Did you notice something? The ratio is coming almost same every time. This ratio is called 'Phi 'which is numerically represented as 1:1.618. This Golden Spiral is made in exact proportion of 1:1.618.


The thing I want to emphasize here is that we always find only those things appealing and attractive that have Phi. It's in our subconscious. Isn't it some revelation? We are the part of this Divine proportion of the God or the Mother Nature. Check these:
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Buzz Chronicles
Buzz Chronicles
@buzz_chronicles
@SaveToBookmarks Hey! Pleasure is mine. Original tweet by @AnJamison: "@TomthunkitsMind @buzz\_chronicles @buzz\\\_chronicles @buzz_chronicles save as ...". Thanks! ✌

Hello! There you go! Original tweet by @SaveToBookmarks: "@AnJamison @TomthunkitsMind @buzz @buzz_chronicles @pdfmakerapp @Readwiseio @thr...". See You soon! 👉
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Save To Bookmarks \uea00
Save To Bookmarks ...
@SaveToBookmarks
@buzz_chronicles @AchuthArora @aldothetrainer @Artofphysique_ @rattibha Gotcha, boss!

Your tweet has been saved for later.

In order to manage your bookmarks you have to join up at savetobookmarks .com. Thanks!
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Sanjeev Upadhyay\U0001f1ee\U0001f1f3
Sanjeev Upadhyay🇮🇳...
@SanjeevUpadhy13
What Happened To Bhakt Prahlad after Hiranyakashipu Vadh
#Thread
Prahlada became the mighty king of the Asuras after Shri Narsimha killed Hiranyakashipu. Prahlada was even more powerful than his father, Hiranyakashipu ever was. He enjoyed the love and respect of his subjects.


By virtue of his good behaviour, Prahlada conquered the three worlds easily and Indra ran away from the Heavens. Indra then deceived Prahlada into giving him the power of his behaviour and Prahlada lost control of the three worlds.


Asuras grew angry at the Devas for taking advantage of their King's virtuous behaviour and invaded the heavens. The Devas, afraid of the Asuras, enlisted the help of human Kings such as Yayati, Raji and Kakutstha and defeated them.

Prahlada attacked the gods and defeated Indra in battle, forcing the King of the Gods to run for his life. Indra sought help of Lord Vishnu. Infused with his power, Indra defeated Prahlada. The latter understood that Vishnu was helping Indra in battle and he withdrew his forces.


Prahlada first gave his kingdom to Andhaka, but the latter was defeated by Shiva. So Prahlada gave it to his son Virochana and undertook a Tirtha Yatra.
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SK
SK
@sk_diary1
A #story from #Thiruvilayadal puranam- one of divine leelas of Bhagwan Shiva

#threadseries #madurai

Koodal Kaandam

Story 21 - it is about how stone elephant came to life and ate sugarcane - When Raja Abhisheka Pandyan met Ellam Valla Siddhar

கல்யானைக்கு கரும்பருத்திய படலம்


Raja Abhisheka Pandiyan went ahead to meet Ellam Valla Siddhar on the premises of the temple.On seeing the arrival of Raja, people surrounding Siddhar moved back. Abhisheka Pandiyan introduced himself to Siddhar &asked about his whereabouts.Also his purpose of visit to Madurai.

Raja asked why he performed magical acts, does he expect anything from him or people of Madurai.

Siddhar laughed & replied to Raja that he was from Kasi. He had heard a lot about Madurai in Kashi.Also told that this place is a Mukti Kshetram,so he had come to stay here.

Siddhar asked Raja if he has the capability &power to give anything that he asks for. Siddhar told King that he has power to do things even if he think of it.Siddhar asked King to ask whatever he wants & it would be provided, whatever these people had asked, they were granted.


Abhisheka Pandyan was shocked to receive such a reply from Siddhar,& he decided to test the power of this Siddhar. By that time, Raja saw a person in crowd having Sugarcane.

Raja took Sugarcane from that person.
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@uglyluhan
First thread of the year because I have time during MCO. As requested, a thread on the gods and spirits of Malay folk religion. Some are indigenous, some are of Indian origin, some have Islamic


Before I begin, it might be worth explaining the Malay conception of the spirit world. At its deepest level, Malay religious belief is animist. All living beings and even certain objects are said to have a soul. Natural phenomena are either controlled by or personified as spirits

Although these beings had to be respected, not all of them were powerful enough to be considered gods. Offerings would be made to the spirits that had greater influence on human life. Spells and incantations would invoke their


Two known examples of such elemental spirits that had god-like status are Raja Angin (king of the wind) and Mambang Tali Arus (spirit of river currents). There were undoubtedly many more which have been lost to time

Contact with ancient India brought the influence of Hinduism and Buddhism to SEA. What we now call Hinduism similarly developed in India out of native animism and the more formal Vedic tradition. This can be seen in the multitude of sacred animals and location-specific Hindu gods
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Greta Musk
Greta Musk
@GretaMusk
🚔 HUGE #Tesla SERVICE PROBLEMS THREAD🚔

You might remember my 3 prior threads on how
TESLA. TREATS. ITS. CUSTOMERS. LIKE. TRASH.

Here's the 4th installment.
@tomkeinan @jonsey_lisa @jwmooc @tchanpoker @bryanthediamond @EconomicManBlog
$TSLA $TSLAQ 1/22


Welcome to Greta's 4th edition of #Tesla customers getting used and abused. Because that's the Elon Musk way.
@coffeebreakls @KocgibiHarun @omaragha @JayGhahari
$TSLA $TSLAQ 2/22


"The best service is no service." - Elon Musk a.k.a. #SpaceKaren
@briansaziz @FarSha998899 @thinktankfour @Billoday1
#Tesla $TSLA $TSLAQ 3/22


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Anu Satheesh \U0001f1ee\U0001f1f3
Anu Satheesh 🇮🇳...
@AnuSatheesh5
Yoga Ramar Temple
#Nedungunam
#TamilNaduTemples 🚩

Yoga Ramar Temple is located near Thiruvannamalai. Shri Rama is seated with eyes closed, without any weapons. Mata Sita  pointing to the feet of Rama telling that  everyone is safe at his feet. Lakshmana standing near Shri Rama


Hanuman is seen chanting Vedas obediently sitting before Shri Rama.

Sthala Puranam says that, Sukabrahma Maharishi had been doing Tapasya here, for the darshan of Sri Rama. Prabhu Shri Ram, pleased with his tapasya, came here after Rama Ravana yudham. Since Rama was returning


after the war, he did not have any weapons with him.

Another gopuram called Kili Gopuram is there for Sukabrahma Maharishi. There is a hill nearby called Dheergachala. According to Sthala puranam, Sri Padham of Prabhu Rama are found on the top of the hill


It is said that this is the place where Shri Rama gave darshan to Suka Maharishi. Nedumgunam means which shows all qualities of Shri Rama who is Maryadha purushottam. Shri Ramanavami is very famous here.

Jai Sri Ram
🙏🕉🙏
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