This nonspecific cation channel opens when illuminated with blue light, depolarizing the cell [4, 6] to trigger action potentials cells with high temporal precision [5]. Since the initial discovery of ChR2, many modifications have been made to refine the kinetic properties.

For example, ChETA, engineered to address limitations of spike fidelity with ChR2, has faster temporal kinetics and can maintain reliable spike precision up to 200 Hz [7].
Halorhodopsins (NpHR), light-gated chloride pumps activated by yellow light, allow for temporally specific hyperpolarization with single spike precision [8–10].
Archaerhodopsin-3 (Arch), a yellow light-activated outward proton pump, is an increasingly popular tool for light-induced hyperpolarization to inhibit cellular activity. Arch recovers spontaneously from inactivation with a much shorter recovery time than NpHRs [11].
Arch currents continue to increase with increasing light intensity, while NpHRs saturate [11]. The increased light sensitivity of the ArchT variant, along with better membrane targeting, allows for improved neural silencing both at cell bodies and terminals [12].
Many more tools have been engineered with unique optical properties and kinetics. Step-function opsins (SFOs) are bistable ChR2s that can maintain a stable open confirmation to induce a step in membrane potential [13].
Opsins with shifted excitation wavelengths allow for differential cellular activation. Chrimson, a red-shifted opsin, can be used in combination with Chronos, a blue-green activated channel with increased light sensitivity and faster kinetics,
to achieve two-color activation of distinct neuronal populations [14]. Since proton pumps are relatively inefficient compared to channels, moving only one ion across the membrane per photon rather than a steady current of ions, to achieve improved neural silencing,
ChR2 was genetically engineered to conduct chloride ions (Cl−) to create a depolarizing channel [15]. Further modifications generated step-function control and improved light sensitivity and kinetics, making these useful tools for controlling behavior in freely moving animals
I will hold my tongue.

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https://t.co/0ywQ1y5NDj

Pott found an association between exposure to soot and a high incidence of scrotal skin cancer in chimney sweeps. This cancer is now known as Pott's or chimney sweep cancer.

Coal contains trace amounts of naturally-occurring radioactive elements. The process of burning coal at coal-fired power plants, called combustion, creates wastes that contain small amounts of naturally-occurring radioactive material (NORM).

Fly ash particles (a major component of coal ash) can become lodged in the deepest part of your lungs, where they trigger asthma, inflammation and immunological reactions.

More from Life

“We don’t negotiate salaries” is a negotiation tactic.

Always. No, your company is not an exception.

A tactic I don’t appreciate at all because of how unfairly it penalizes low-leverage, junior employees, and those loyal enough not to question it, but that’s negotiation for you after all. Weaponized information asymmetry.

Listen to Aditya


And by the way, you should never be worried that an offer would be withdrawn if you politely negotiate.

I have seen this happen *extremely* rarely, mostly to women, and anyway is a giant red flag. It suggests you probably didn’t want to work there.

You wish there was no negotiating so it would all be more fair? I feel you, but it’s not happening.

Instead, negotiate hard, use your privilege, and then go and share numbers with your underrepresented and underpaid colleagues. […]

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