2. PREEMPT lab teams
https://t.co/GOUGRoRGfI * Autonomous Therapeutics, Dr. Ariel Weinberger
* One Health Institute UCD, Drs. Peter Barry & Brian Bird
* Institut Pasteur, Dr. Carla Saleh
* Montana State University, Dr. Raina Plowright
* Pirbright Institute, Dr. Luke Alphey
3. Identifying Suspect Bat Reservoirs of Emerging Infections
https://t.co/lZPBAIjkm7 Defense Advanced Research Projects Agency (DARPA D16AP00113), and DARPA PREEMPT program Cooperative Agreement (DARPA D18AC00031).
4. Peng Zhou, Linfa Wang, Zhengli Shi, Lijun Wu (WIV)
2104 (CSIRO Australia)
IRF7 in the Australian Black Flying Fox, Pteropus alecto: Evidence for a Unique Expression Pattern and Functional Conservation
https://t.co/UzpI9r0uA7
5. Peng Zhou, Linfa Wang, Zhengli Shi
(Sep 2011 - CSIRO Australia)
Type III IFN Receptor Expression and Functional Characterisation in the Pteropid Bat, Pteropus alecto
https://t.co/1S6OrRrtfK
6. ¿Cómo encontrar el origen de los coronavirus?
Qiu, Jane
.Investigación y Ciencia; Dordrecht (Jun 1, 2020).
https://t.co/taZoyHxrqR and
https://t.co/8FtSri34PX
7. BVLPs - Bat Covs + SARS Cov - Baculovirus (WIV 2008)
VLPs of SARS-Like Coronavirus Formed by Membrane Proteins from Different Origins Demonstrate Stimulating Activity in Human Dendritic Cells
https://t.co/eRV8lSyf2P Bai, Bingke; Hu, Qinxue; Hu, Hui; Zhou, Peng; Shi, Zhengli;
8. Above paper cited by 7 in Pro Quest
https://t.co/8Jr9lCSZu4
9. Immunogenicity of spike glycoprotein of Bat SARS-like coronavirus
https://t.co/3PQkkYHSjQ Insect cell technology - versatile & robust vaccine manufacturing platform
https://t.co/d8WKHrXz0V VLP-based vaccines against emerging infectious disease viruses
https://t.co/gSVag3xAul
10. Insect Cell Technology & Vaccine Development
Paper in Tweet 9 is a useful 2014 review of vaccines based on baculovirus systems with 5 tables showing all vaccine projects for humans & animals
https://t.co/uDjmxl7sZD Tables 1, 2, 3 shown here
Text only:
https://t.co/VRlG1itgDY
11. Tables 4 and 5
Table 4. Baculovirus-expressed virus-like particles for veterinary immunization
Table 5. Baculovirus vaccines under development.
Text version:
https://t.co/VRlG1itgDY CSVs:
https://t.co/uDjmxl7sZD Only a few coronavirus vaccines mentioned, next tweet
12. WIV Baculovirus based Cov Vaccine Project (1)
1. WIV 2008 - Mice- Insect Cells - Bat Covs- Sars
Vaccination of mice with recombinant baculovirus expressing spike or nucleocapsid protein of SARS-like Cov generates humoral & cellular immune responses
https://t.co/bTn9INxo1w
13. SARS-HIV Baculovirus based Cov Vaccine Project (2)
Peking (2006)
Baculovirus surface display of SARS coronavirus (SARS-CoV) spike protein and immunogenicity of the displayed protein in mice models
https://t.co/RjrMb0YYZG full text:
https://t.co/iQYr3GDq1z
14. Previous paper used SARS-HIV technique based on work in 2004 by Peking Uni Researchers
Identification of Antigenic Determinant on S2 Domain of Severe Acute Respiratory Syndrome Coronavirus Spike Glycoprotein Capable of Inducing Neutralizing Antibodies
https://t.co/dNh985mjMA
15. This HIV-SARS technique was based on paper (Ref 46) (2004)
Expression cloning of functional receptor used by SARS coronavirus
https://t.co/q5halBhjFa "ACE2 mediated entry of HIV/SARS pseudovirus & goat anti-human ACE2 polyclonal antibody abrogates entry of HIV-luc/SARS"
16. The above was partly based on similar research in 2004:
Highly infectious SARS-CoV pseudotyped virus reveals the cell tropism and its correlation with receptor expression
https://t.co/bBIWIHMgn2
17. This research ties in with another previous thread I did on the so-called HIV inserts, vaccine development, horizontal gene transfer, contamination and VLPs:
https://t.co/P7QbDEBG52
18. That thread's main focus is on possibility of HIV artefacts appearing in SARS-COV-2 through horizontal gene transfer during vaccine development using HIV vlps and SARS spike proteins
https://t.co/G39nSzmqmj
19. Enough, time for a break
https://t.co/JlAPbg25Zb